BioProduct : Cell Biology : Tissue & Cell Culture : Cell & Tissue Culture Growth Factors : mRNA Catcher™PLUS Kit
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Product Name mRNA Catcher™PLUS Kit
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Product Description "The mRNA Catcher™PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher™PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity. The mRNA Catcher™PLUS kit offers:   •   Robust performance - high binding capacity per well (>80 ng/well)   •   Reproducible results - low CVs from well-to-well and plate-to-plate (~2%)   •   Optimized protocols for - small sample sizes (100 cells, 4 mg tissue, and 40 µl blood)   •   Compatible plate design - may be used with most PCR cyclersHow it worksthe mRNA Catcher™PLUS Kit, mRNA from total RNA, cells, tissue, and blood samples are hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher™PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.

The mRNA Catcher™PLUS Kits include Lysis Buffer, Wash Buffer, Elution Buffer, Dilution Buffer, plates, and DTT. Store at room temperature. Guaranteed stable for 6 months when properly stored. "
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Specification960 reactions
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Vendor Information
Life Technologies
5791 Van Allen Way
CA 92008

Phone: (760) 603-7200
Fax: (760) 602-6500