BioProduct : Molecular Biology : Cloning & Expression : cDNA Cloning : SuperScript™ Mouse 8.5 Day Embryos cDNA Library (male C57BI : 6J)
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Product Name SuperScript™ Mouse 8.5 Day Embryos cDNA Library (male C57BI/6J)
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Product Description "SuperScript® Pre-made cDNA Libraries are suitable for isolating novel and published cDNAs, PCR of target sequences, and gene expression in eukaryotic cells (1). Each library is constructed using SuperScript® II reverse transcriptase for   Morefirst-strand synthesis to generate full-length and high-yield cDNA. The libraries have a large average insert size, typically 1.3 to 2.5 kb, ensuring high representation of full-length clones. Each library has greater than 5 x 109 cfu derived from>107 primary clones to ensure complete representation of even the rarest sequences. Libraries are amplified only once using a semi-solid amplification process for faithful representation of the primary library (2). Libraries are directionally cloned into the eukaryotic expression vector pCMV•SPORT containing a CMV promoter for expression analysis, an f1 ori for single-strand DNA production, and SP6 and T7 RNA polymerase promoter sites flanking the MCS for RNA synthesis. Libraries may be screened using conventional PCR or plate screening procedures, or by functional analysis using the eukaryotic CMV promoter."
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Life Technologies
5791 Van Allen Way
CA 92008

Phone: (760) 603-7200
Fax: (760) 602-6500