BioProduct : Molecular Biology : Nucleic Acid Labeling : OliGlo™ Biotin FISH Kit - standard size
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Product Name OliGlo™ Biotin FISH Kit - standard size
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Product Description Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence similarity. Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosome. FISH is often employed for discovery of specific features in DNA and is useful in genetic analysis, medicine, or species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples. In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues. The probes used in these analyses are typically short complimentary sequences to the target DNA which can be fluorescently labeled using this kit. In a typical protocol, metaphase chromosome spreads are prepared on glass slides, RNase treated, dehydrated and denatured. The slides are then hybridized with the fluorescently labeled DNA probes for 16-24 hours. Post hybridization, the slides are washed, counterstained with a 4’,6-diamidino-2-phenylindole (DAPI) / antifade mixture, and analyzed using fluorescence microscopy. Our OliGlo™ kits directly label nucleic acid through reaction with the phosphate groups (terminal and backbone) of the probe nucleotide providing cleaner hybridization analysis. Application: In our OliGlo™FISH kit, the active labeling reagents are prepared in situ from stable precursor molecules derived from a variety of highly fluorescent dyes and other detection labels. This allows the highly reactive labels to function at optimum efficiency for each sample. The supplied standard labeling protocol will yield labeling efficiency of approximately 10 to 100 labels per kilobase of nucleic acid depending on the properties of the different labeling dyes and purity of the sample. We found that this labeling density is sufficient for most FISH applications. Should there be a need for adjusting labeling efficiency, the user can simply modify the ratio of labeling dye to nucleic acid as well as incubation times for the labeling reaction as necessary.
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Price $720
Specification1 kit
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Vendor Information
Marker Gene Technologies, Inc
1850 Millrace Dr
OR 97403
United States

Phone: 5413423760
Fax: 541 342 1960