BioProduct : Tissues & Cell Lines : Human Cell Lines : Human Normal Cryopreserved Non-Parenchymal Primary Cells (HNCNPC)
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Product Name Human Normal Cryopreserved Non-Parenchymal Primary Cells (HNCNPC)
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Product Description BioSafety Level II Organism Homo sapiens Source Organ Liver Growth Properties Adherent Morphology Polygonal Propagation The base medium for this cell line is Prigrow III medium available in ABM, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin(G255). This cell line needs to be cultured in collagen coated flasks. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Subculturing 1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37C to facilitate dispersal. 3. Centrifuge cells at 1500rpm for 3 minutes to pellet. 4. Aspirate out trypsin, leaving pellet undisturbed. 5. Resuspend pellet in fresh culture medium and plate in new collagen coated culture vessel. 6. Incubate cultures at 37C. Preservation 1. Freeze Medium: Complete growth medium with 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase. Notices and Disclaimers 1. ABM products are intended for laboratory research purposes only, unless noted otherwise. They are not intended for use in humans. 2. ABM makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. ABM does not warrant that such information has been shown to be accurate.
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Price $525.00
Specification1 vial, 10^6 cells
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Vendor Information
Applied Biological Materials Inc. 
http://www.abmgood.com
13520 Crestwood Place - Suite 8
Richmond
BC V6V 2G2
Canada

Phone: 1-604-247-2416
Fax: 1-604-247-2414
E-Mail: info@abmgood.com