BioProduct : Protein Chemistry : Protein Kits : MaxDiscovery™ Aspartate Transaminase (AST) Color Endpoint Assay Kit
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Product Name MaxDiscovery™ Aspartate Transaminase (AST) Color Endpoint Assay Kit
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Product Description The MaxDiscovery™ Aspartate Transaminase (AST) Color Endpoint Assay Kit is a plate-based colorimetric enzymatic assay for the determination of the aspartate transaminase enzyme in serum samples. Aspartate transaminase (AST) also known as aspartate aminotransferase or (sGOT) is a metabolic enzyme expressed primarily in the liver. Elevation of AST levels is an indication of liver damage and has been associated with liver injury. AST levels are monitored routinely in patients with liver diseases. AST is also a very useful tool for preclinical investigation of experimental drug formulations and AST levels are commonly used to monitor and attenuate the hepatotoxic effects of experimental drugs in rodents. The MaxDiscovery™ Alanine Transaminase (AST) Color Endpoint Assay Kit uses a colored reaction scheme to detect AST enzymatic activity in serum samples. In this method aspartate and á-ketoglutarate are first converted to glutamate and oxaloacetate. The oxaloacetate then reacts with diazonium salt to form a colored product. The concentration of AST in each sample is then directly determined from the absorbance at 510 nm measured with a plate reader. Dilutions of the oxaloacetate control, included in the kit, can be used to construct a standard curve to calibrate the assay and confirm assay linearity.
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Price $250
Specification1 x 96 wells
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Vendor Information
Bioo Scientific
3913 Todd Lane
Suite 312
TX 78744

Phone: 1-888208-2246