Washing cuvettes with 0.1M NaOH, 1 mM EDTA - (Apr/13/2005 )
According to my protocol; "When measuring RNA samples, be certain that cuvettes are RNase-free, especially if the RNA is to be recovered after spectrophotometry. This can be accomplished by washing cuvettes with 0.1M NaOH, 1 mM EDTA followed by washing with RNase-free water"
What does it mean with "0.1M NaOH, 1 mM EDTA2" ? Are they in the same solution or should i wash first with 0.1M NaOH solution and then after that i wash with 1 mM EDTA solution?
Thanks.
-searcher-
It is one solution containing both.
-pcrman-