SP-sepharose column - can this make column "out of order" ? (Apr/12/2005 )
at the work with a SP-Sepharose column chromatography , proteins stick tightly to column and do not elute with the salt gradient .
even with the 1.5M NaCl some proteins remain in the column and we have to wash the sp sepharose with 1M NaOH . can this make the column "out of order" ?
what should I do?
-gholi-
QUOTE (gholi @ Apr 12 2005, 02:14 AM)
at the work with a SP-Sepharose column chromatography , proteins stick tightly to column and do not elute with the salt gradient .
even with the 1.5M NaCl some proteins remain in the column and we have to wash the sp sepharose with 1M NaOH . can this make the column "out of order" ?
what should I do?
even with the 1.5M NaCl some proteins remain in the column and we have to wash the sp sepharose with 1M NaOH . can this make the column "out of order" ?
what should I do?
According to the manual of resin of Pharmacia, the sp-sephorose can be cleaned with 1M NaOH.
-dowing-