Hi,
This may seem liek bit of a trivial question but Im trying to make a buffer to wash and store my cells, and the protocol I'm using states that I should dissolve 30g in 1litre of water, but I can not get this amount to dissolve. I was just wondering if anyone had any ideas to why this may be? Thanks.

Hey,

EDTA needs a certain pH to dissolve completely. So you have to make sure your buffer has the right pH-value before dissolving EDTA.

I think it is something around 8-10...not sure!

Try to find out which pH is needed and then it should work.
Sorry, for not remembering the pH-value in detail.
Good luck
Cheers

I agree with Bomber...EDTA is not soluble at acidic pH; it is necessary to add alkali gradually and to control pH ;the pH can be adjusted with acetic acid or NaOH. So adjust pH 8.0 with 10 M NaOH ..Once pH is OK, it will be dissolved..
Hope it helps...

hi
well, EDTA can not dissolve under a pH very close to 8. It's a craezy solution to prepare...
I mako solution at 500mM usually, and disolution occurs at 7,95!
fred

yup, whenever i make a stock of this i use NaOH pellets and put them in while stirring. I added about 10 of them and i only had to pH a little bit afterwards....worked like a charm!


~QG