Murine blood extraction technique - (Feb/04/2005 )
if i use retro- orbital punction, will the mouse die?
Not if you do it well, don't take too much blood etc.
b3ka
if i use retro- orbital punction, will the mouse die?
Not if you do it well, don't take too much blood etc.
b3ka
Take no more than 0.4ml.
if i use retro- orbital punction, will the mouse die?
Not if you do it well, don't take too much blood etc.
b3ka
Take no more than 0.4ml.
ok, thanks
Another fast and easy way to bleed unanesthetized mice and rats is the saphenous vein. You will be able to collect the 1% of the body weight sample as easily as retro-orbitals . . .
After carbon di oxide anaesthesia I am willing to take maximum blood at one shot from every mice and using BD EDTA sprayed tubes to store for further proteomics which experiments yet to be planned. Please let me know if any suggestions and advice before starting the experiments. Thanks
I am also interested to pool urine ( I know I will take long) but I want to give full best to collect urine to have proteomics done. Kindly let me know any ideas regarding as well.
Rgds
After carbon di oxide anaesthesia I am willing to take maximum blood at one shot from every mice and using BD EDTA sprayed tubes to store for further proteomics which experiments yet to be planned. Please let me know if any suggestions and advice before starting the experiments. Thanks
I am also interested to pool urine ( I know I will take long) but I want to give full best to collect urine to have proteomics done. Kindly let me know any ideas regarding as well.
Rgds
hmm, pooling the urine is tough. we looked at that here.
there are a couple of options - cytocentesis isn't very effective. as we all know, mice are perpetually peeing 
their bladders just aren't large enough to hold much. we tried that several ways and couldn't get more than a few ul per mouse
however, there are two things that work?
euthanize them in a cage with no bedding. they pee, then you take it out with a pipette. you can get ~20-50ul per mouse this way at the time of death.
house them in a metabolic cage with no bedding. you can collect their urine over time this way until there is enough for your purposes. I haven't tried this (we were able to get enough the other way), but I hear you can get a few mL over several days from a cageful