What is the difference between 2x and 4x sample buffer? - SDS Sample buffer (Mar/01/2002 )
Hi
I'm running SDS gels, I want to make up a 4x sample buffer.  Do I just make up the 2x recipe in half the final volume?
thanks
-AmyM-
here's a recipe for 4x from gibco
4x Sample buffer for (non-reduced gel): (for 10 mls use) 
       12% SDS              1.2g 
       40% Glycerol        4.0 ml 
       0.2 M tris-HCl pH7  2.0 ml of 1 M Stock 
       0.004% Bromophenol Blue  0.4 mg 
 4x Sample Buffer (for reduced gel): 
 Same volumes and amounts as #1 above but add to 
 solution 0.5 ml 2-Mercaptoethanol.
-jamescastle-
