How to find the transcription start site of an unknown gene? - (Nov/03/2004 )

How to find the transcription start site of an unknown gene?

how many method??

Please email to :

sallymmxhl@yahoo.com

Thanks everyone!

Hi Sally,

One quick method is to search and align all EST's you can find for the gene in genbank. If you find 2 or 3 in the most 5' end that initiate at the same base, it is probably the startsite.

regards,

Søren

Søren M. Echwald, MSc., Ph.D.
-----------------------------
Exiqon A/S
Bygstubben 9
DK-2950 Vedbaek
Denmark

www.ProbeLibrary.com
One Real-time PCR kit, which covers 38.565 genes