Autoclaving Glucose - (Nov/12/2008 )
edit: Gosh!! I forgot I was in another tab. Could the moderators help out? I'm sorry.
I've been cautioned against autoclaving glucose as they'd caramelize. Now, looking up on it I found differing method in sterilizing it. 1) autoclaving it separately 2) sterilized together with the MR-VP medium
So, has anyone sterilized their stock solution of glucose before? Will 50% v/v be fine? More? Less?
Thanks in advance!
Why not filter your solution with a .22 µm pore?
I autoclave 20% glucose, 121C/15min for use in plates to grow yeast and I dont have any problems with that.
For the time being I don't have the membranes with me and I need the glucose next week so I thought of sterilizing them this way. I need quite a high concentration of glucose 40-50% v/v so am not sure if that high a concentration would cause it to brown.
you can do 40%, a guy in my lab does and it does not go brown.
Will do. Thanks all.