How to get rid of PCR primer dimer - (Mar/17/2003 )

Hi, medchemgirl...

I'm noreen..Ive been doing RT-PCR works n my PCR doesn't turn out to be so good..I've come across your comment n u r having gud results using DMSO..what are the concentration of the DMSO n when did u put it in the pcr reaction..thanks..

Hi guys!
I am facing the same problem of primer dimer....I have designed my primers recently for Mycoplasma detection, but the primers have different annealing temp and also dont find any bands at expected bp length?

am new to this forum! so any suggestions are welcome

There is a product that I use called PCRboost. Its from a company called Biomatrica. I just put this in my PCR reactions that I want better results with. Its great. I ran a comparison side by side and I can see a very noticable increase. It also helps with primer dimers, so I thought it might help you with your problem as well. Hope this helps!

Austin