Dear All,
I'm new in the field and I have a commercial kit to measure hGH. To get the results, I need to measure the absorbance of the samples at 405 nm with a reference wavelength at 490 nm. Does that mean that I have to do 2 measurements of the same plate than remove the lower value from the higher value to get my results ? or is it something else that I don't understand????
Sorry to be so stupid
Tks

That means when you setup the parameter for your reader, you have to use two channels, one is 405 nm, another as reference at 490 nm. The reader will automatically adjust results when done.

Dear All,
I'm new in the field and I have a commercial kit to measure hGH. To get the results, I need to measure the absorbance of the samples at 405 nm with a reference wavelength at 490 nm. Does that mean that I have to do 2 measurements of the same plate than remove the lower value from the higher value to get my results ? or is it something else that I don't understand????
Sorry to be so stupid
Tks

Hi !
Thank you for this reply. My reader can't use 2 channels at he same time (it's an old machine ^^), so I do 2 measurements one at 405 the other at 490 and then I subtract manually; would that be more or less correct ?

Hi,
if your machine cannot filter two wavelengths at the same time, you can measure individually and substract manually
all the best
Leelaram