No bands observed on anti-His western blot - (Jul/31/2008 )
QUOTE (RandomGuy187 @ Aug 6 2008, 11:28 PM)
I also tried 8 M urea, which didn't do anything either.
Still you didn't answers my little questions about the sequence Of course they might other explanations Protein expression is a tough business
boiling for 20min at 100C may be too long (can cause protein to aggregate). 3min should be sufficient.
if you warm at 65C then you can use 10 or more minutes.