dried SDS-PAGE - (Jan/24/2008 )

hi friends

is there any method to dry SDS-PAGE (8-10%) without breaking
not a special tool but I wonder that it is possible by the way heating in an etuv or others

thanks

fatoş

---

There are some apparatus that dry by heating under vacuum. Fast and convenient
If you want to scan your gel and have a nice picture I would recommend you something like gelair dryer. It takes more time, and you need to incubate the gel in water plus 10% metOh and 3% glycerol to avoid gel cracking. But the dried gel is beautiful.
Have a look on biorad webpage :
http://www.bio-rad.com/B2B/BioRad/product/...cience+Research

---

if you have no gel dryer, you may do the old method with using household preservation membrane (ususally for preservation of vegetable, fruits or jam); it must tightly be mounted in a frame and dried by air

---

I usualy put the gel in a 30% etOH solution with a bit of glicerol (not sure about the concentration though, I just pour a couple of mLs). I leave it in that solution overnight and then put the gel on a glass plate and cover it with what we call acetate paper. I really don't know the name in english... It's basically those transparent sheets, kind of like gled. In a couple of days the gel is fully dry.

---

Hi,

Cellophane sheets to dry.

---