2007 )

Does anyone know at what wavelength EtBr will absorb? or will it flouresence? (after binding to RNA so I can measure the amount of RNA)

THanks

-teresachang83-

The lab rat

has a good protocol, basically in the presence of EtBr nucleic acids (DNA/RNA) excited at 546 nm, will emit light at 590 nm.

So you need a Fluorometer

sadly it wont distiguish between RNA /DNA

Most dyes have this problem but Invitrogen now sell there Ribogreen with DNAse so that you can digest away the DNA first

Ian

-DevGrp-

Out of curiosity...
Have you isolated RNA? If so, are you worried about DNA contamination? If you've extracted RNA (using a kit or phenol:choloroform), you can use absorbance at 260nm to quantitate RNA.

-NPMALK-