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DNA loss in digestion - I lose lot of DNA in digestion (Aug/06/2007 )

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QUOTE (scolix @ Aug 8 2007, 02:15 PM)
Any hint of a smear in the gel? Could you try to change the elution buffer when you purify from the gel. It may contain some contamination which might work efficiently in the presence of the enzyme buffers and result in loss of DNA. its just a guess.

Good Luck !!!

NO smear ..very clear dark bands...


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