can blunt & sticky ends stick - (Jul/03/2007 )
well, you could increase the ratio of insert to vector and increase the concentration of vector and insert in the ligation mix.
I would also suggest a review of what you are actually doing. The devil is in the detail.
-perneseblue-
well, you could increase the ratio of insert to vector and increase the concentration of vector and insert in the ligation mix.
I would also suggest a review of what you are actually doing. The devil is in the detail.
-perneseblue-