Urgent amp agar plate question! - Help with stupid question (Jun/20/2007 )
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satellite colonies are small colonies which grow around the "true" colonies containing the resistance gene. they are non-resistant colonies which grow in a circular pattern around the resistant ones. Resistance is mediated through beta-lactamase, which is produced by the resistant colony in the center. therefore satellite colonies where able to grow on place where the ampicillin is degraded (around the resistant colony).
I found a picture: http://www.gbiosciences.com/uploaded_descfiles/amp47_b.gif
QUOTE (Krisztina @ Jun 22 2007, 07:43 PM)
What is a satellite colony and how do they look like?
Thanks.
Thanks.
satellite colonies are small colonies which grow around the "true" colonies containing the resistance gene. they are non-resistant colonies which grow in a circular pattern around the resistant ones. Resistance is mediated through beta-lactamase, which is produced by the resistant colony in the center. therefore satellite colonies where able to grow on place where the ampicillin is degraded (around the resistant colony).
I found a picture: http://www.gbiosciences.com/uploaded_descfiles/amp47_b.gif
-moljul-
When I used to cloned several years ago what I did to have fast ready plates was to prepared several bottles with the media (about 125mL) autoclaved them and stored with parafilm at 4%, when needed I just melt them in a double boiler, wait until cool down, add the antibiotic and plate them.
-merlav-
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