gel electrophoresis - problem with stacking (Jan/18/2007 )
Pages: 1
don't use the Same PH 8.8 buffer for the stacking , make sure that you made the 10% APS fresh in distilled water , you can increase the TEMED which will solve your problem , or alternatively you can use 5% stacking gel,
QUOTE (biotech! @ Jan 18 2007, 08:46 PM)
Hi, does anyone know why it can be happening this: When doing gels I have no problem with the polimeration of the gel from below but, the stacking keeps liquid or takes hours to be made. ![mad.gif]()
......it's really weird since I use exactly the same things that for the first one except for the buffer (pH 6.8 instead of 8.8).
Now I found a solution....I use the same pH 8.8 buffer for both parts and it works.......
I' don't know what might be happening, I'already checked the pH so that's not the reason.
By the way, do you know why the buffers have to have different pH??![blink.gif]()
well, hoping to find the reason!! ;)
Now I found a solution....I use the same pH 8.8 buffer for both parts and it works.......
I' don't know what might be happening, I'already checked the pH so that's not the reason.
By the way, do you know why the buffers have to have different pH??
well, hoping to find the reason!! ;)
don't use the Same PH 8.8 buffer for the stacking , make sure that you made the 10% APS fresh in distilled water , you can increase the TEMED which will solve your problem , or alternatively you can use 5% stacking gel,
-Amit nayak-
Use only fresh APS and mix well the mixture
QUOTE (biotech! @ Jan 18 2007, 04:16 PM)
Hi, does anyone know why it can be happening this: When doing gels I have no problem with the polimeration of the gel from below but, the stacking keeps liquid or takes hours to be made. ![mad.gif]()
......it's really weird since I use exactly the same things that for the first one except for the buffer (pH 6.8 instead of 8.8).
Now I found a solution....I use the same pH 8.8 buffer for both parts and it works.......
I' don't know what might be happening, I'already checked the pH so that's not the reason.
By the way, do you know why the buffers have to have different pH??![blink.gif]()
well, hoping to find the reason!! ;)
Now I found a solution....I use the same pH 8.8 buffer for both parts and it works.......
I' don't know what might be happening, I'already checked the pH so that's not the reason.
By the way, do you know why the buffers have to have different pH??
well, hoping to find the reason!! ;)
-biomolman-
Pages: 1