mouse CD19 promoter sequence - (Dec/19/2006 )
Hi,all friends
Can anyone tell me the sequence of mouse CD19 promoter ? I really need it to construct a transgene vector which will be specifically expressed on B cells.Thanks a lot
Qing
-qingding-
CODE
GTCCCTTTCTTTGGGGGGTGGAGGGTAGGGTGGGATTGAGACAGATTCTCACTATGTAGC
CATGGCTGGCCTGAAACTTACAGCGATCCACTTGTTGCTGCCTCCCAGTTGCTGGGATTA
AAGGTGTGTGCCACTCTGCCTGTTTGGCTCTTTCTTTTTTGAGGGGGAGGTCTTGCTATA
TAGTCTAAGCTAGAATGAAACCTAAAATGTGTGTGTGTTTGTGTGTGTGTGTCTGTGTGT
GTGTGTGTGTCTGTCTGTCTGTCTGCGTCTATGTCTGTGTGGTAGGAGGGATTAAATCTA
GGGTCTTGTATATACTTTGGCACTGAACTATGTCCTCAACTTTTTAAAAAAGTATTTTTT
AATTTTCAGATAAGGTCTCCCTGAGTACTTTAGAGTGGCTTTGAACTCACTCTGCAGTCC
AAGCTGGCCTTGACTTTTGTGATCCTCTCCAGTAACTGGAATTATAAGCCTGGATCACCA
GACCCAATATAAAAATCCTAATGCTATCCCCAGATGATAGAGGAAAAAAAGCGGATGCCA
GACATTGTGGCATATGCCTTTAACTTCACCAGCTGGGAAACAGAGGTGTAAAATAAGGTC
AGAGCCTGGAGCCTGGAGAGAGGAGGAGAAAGAAAACCCTAACTTAAGCCTGCACCCACT
CATCTGAAAAATATTTAACAGGTGCCACTATGTGGAAAGCTCTGTGTGTGCAGTAGTGAA
GATGTTAGCCTTACCCGGAGGAACTCCCATGCCAAACCATATAGAAGGTCACACACTTGG
GCTAAGTGCTGGGTGACAGGGAGGGGGACAAGTGTTGTGGATTTGGAAGAGTGCCTGCAA
ATGGAGGCCTAACCTAAGGTGTGACCACCCCTTTCCTCTATACGGGGACTGCCACCACCC
TCACAGACACCCATGGTTCATTGCCCACTAGGCTCCTGCTCGCCCCAGCATCCTCTGCGC
AGCCACGCAGAAGCCGGGTGCCCCAGGGAGCCTGGCTACC[+1]ATGCCATCTCCTCTCCCTGT
CATGGCTGGCCTGAAACTTACAGCGATCCACTTGTTGCTGCCTCCCAGTTGCTGGGATTA
AAGGTGTGTGCCACTCTGCCTGTTTGGCTCTTTCTTTTTTGAGGGGGAGGTCTTGCTATA
TAGTCTAAGCTAGAATGAAACCTAAAATGTGTGTGTGTTTGTGTGTGTGTGTCTGTGTGT
GTGTGTGTGTCTGTCTGTCTGTCTGCGTCTATGTCTGTGTGGTAGGAGGGATTAAATCTA
GGGTCTTGTATATACTTTGGCACTGAACTATGTCCTCAACTTTTTAAAAAAGTATTTTTT
AATTTTCAGATAAGGTCTCCCTGAGTACTTTAGAGTGGCTTTGAACTCACTCTGCAGTCC
AAGCTGGCCTTGACTTTTGTGATCCTCTCCAGTAACTGGAATTATAAGCCTGGATCACCA
GACCCAATATAAAAATCCTAATGCTATCCCCAGATGATAGAGGAAAAAAAGCGGATGCCA
GACATTGTGGCATATGCCTTTAACTTCACCAGCTGGGAAACAGAGGTGTAAAATAAGGTC
AGAGCCTGGAGCCTGGAGAGAGGAGGAGAAAGAAAACCCTAACTTAAGCCTGCACCCACT
CATCTGAAAAATATTTAACAGGTGCCACTATGTGGAAAGCTCTGTGTGTGCAGTAGTGAA
GATGTTAGCCTTACCCGGAGGAACTCCCATGCCAAACCATATAGAAGGTCACACACTTGG
GCTAAGTGCTGGGTGACAGGGAGGGGGACAAGTGTTGTGGATTTGGAAGAGTGCCTGCAA
ATGGAGGCCTAACCTAAGGTGTGACCACCCCTTTCCTCTATACGGGGACTGCCACCACCC
TCACAGACACCCATGGTTCATTGCCCACTAGGCTCCTGCTCGCCCCAGCATCCTCTGCGC
AGCCACGCAGAAGCCGGGTGCCCCAGGGAGCCTGGCTACC[+1]ATGCCATCTCCTCTCCCTGT
Here is the 5' flanking sequence (1kb) of mouse CD19. The last 20 nts are first exon.
The sequence doesn't have CpG island. Promoter prediction result using NNPP program
QUOTE
Start End Score Promoter Sequence298 348 0.92 CTAGGGTCTTGTATATACTTTGGCACTGAACTATGTCCTCAACTTTTTAA
477 527 0.81 ACCAGACCCAATATAAAAATCCTAATGCTATCCCCAGATGATAGAGGAAA
514 564 0.94 ATGATAGAGGAAAAAAAGCGGATGCCAGACATTGTGGCATATGCCTTTAA
579 629 0.99 AACAGAGGTGTAAAATAAGGTCAGAGCCTGGAGCCTGGAGAGAGGAGGAG
663 713 0.82 TCTGAAAAATATTTAACAGGTGCCACTATGTGGAAAGCTCTGTGTGTGCA
749 799 0.87 ATGCCAAACCATATAGAAGGTCACACACTTGGGCTAAGTGCTGGGTGACA
869 919 0.84 CCCTTTCCTCTATACGGGGACTGCCACCACCCTCACAGACACCCATGGTT
477 527 0.81 ACCAGACCCAATATAAAAATCCTAATGCTATCCCCAGATGATAGAGGAAA
514 564 0.94 ATGATAGAGGAAAAAAAGCGGATGCCAGACATTGTGGCATATGCCTTTAA
579 629 0.99 AACAGAGGTGTAAAATAAGGTCAGAGCCTGGAGCCTGGAGAGAGGAGGAG
663 713 0.82 TCTGAAAAATATTTAACAGGTGCCACTATGTGGAAAGCTCTGTGTGTGCA
749 799 0.87 ATGCCAAACCATATAGAAGGTCACACACTTGGGCTAAGTGCTGGGTGACA
869 919 0.84 CCCTTTCCTCTATACGGGGACTGCCACCACCCTCACAGACACCCATGGTT
-pcrman-
Hi,
just read the latest post in topic:
""../../forums/inde...showtopic=16935
-Markus Germany-