Hi everyone,
I have done H.E staining for liver tissue which had been fixed with 3% paraformaldehyde after removed from the mouse for several times, but the pictures showed that the normal liver doesn't look normal. My boss said that the reason is the fixation is not good. Does anyone use paraformaldehyde to fix liver? How do you do that?
Thanks in advance.

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You need to perfuse liver with fixative through Vena Cava with hepatic vein cut. 5 ml is enough.

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Thanks. But I can not fix the whole liver, because I still need use the liver tissue to isolate hepatocytes.

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Try to cut it into small blocks with a double edged razor blade (0.5 cm x 0.5 cm or less, from the edge of a lobe, to allow better penetration of fixative). Dip it in cold 4% paraFA in PBS. When tissue is harderned in 3-5 min, further cut it into smaller blocks of 1/2 to 1/4 the original size.

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Try to cut it into small blocks with a double edged razor blade (0.5 cm x 0.5 cm or less, from the edge of a lobe, to allow better penetration of fixative). Dip it in cold 4% paraFA in PBS. When tissue is harderned in 3-5 min, further cut it into smaller blocks of 1/2 to 1/4 the original size.
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Thanks a lot. That will be very helpful. I will try this.

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