"Genome Replication, Virion Secretion, and e Antigen Expression of Naturally Occurring

Hepatitis B Virus Core Promoter Mutants
Sameer Parekh,1 Fabien Zoulim,2 Sang Hoon Ahn,1 Adrienne Tsai,1 Jisu Li,1 Shigenobu"



in figure 6

he separated core particles from Dane particles through CsCl gradient. Indeed, clones such

as 4B, 5.2, and 6.2 released two types of particles, with a double-stranded genome inside

lighter Dane particles and a single-stranded genome inside heavier core particles


but in figure6 first light particles appeared , but as we know when we isolate fraction

heavier components apear first .how lighter dane particle appear first




please answer

i haven't seen the article and, therefore, haven't seen the figure but i would guess that they are showing fractions taken from the top of the gradient. one of the methods for fractionating a cscl gradient is to introduce a heavy solution to the bottom of the tube and to float the cscl out of the top of the tube.

thank u very much i v got it