hi


in the following line

"For HBV transcription to proceed under the control of endogenous core promoter, tandem dimers were made."


what is the advantage (except large replication products) of inserting two sequences in puc18.does single sequence is not sufficient?

without any context it's very difficult to answer.
maybe they wanted the molecule to be a dimer. some time, a repeated sequence is better recognized by T helpers, for example.

for studying hbv replication in one article author made following strategy

"HBV replication can proceed (to various extents) from different DNA constructs, including vector-free circularized or linear genome (16), vector-linked tandem dimer (52), and a DNA copy of pregenomic RNA under foreign (cytomegalovirus) promoter, we selected to pursue the tandem dimer approach. Such a DNA form allows HBV replication to proceed under the endogenous core promoter rather than an artificial cytomegalovirus promoter and thus permits the expression of HBeAg. This turned out to be judicious, since all of the high-replication genomes identified in the present study are core promoter mutants. Core promoter mutations modulate HBV replication and HBeAg expression at the transcriptional level"

my question is why only "single hbv sequence" not enough .why two sequences are inserted


thanx for reading

I think perhaps you may find the answer yourself

if you look on pubmed or google and type in 'tandem dimer hbv' or even just 'tandem dimer' you get many references using/discussing this technique.

another thing, have you gone to ref #52 and looked up that paper? perhaps it is better explained.

i am searching for long time the reason "why" dimer is construct .does single sequence is not enough for expression.