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Cotransfection - (Feb/20/2002 )

I am planning to do a stable cotransfection in PC12 cells.
How could I set a double selection? Is it better to start with one transfection, do the selection and then carry on with te second transfection/selection or what?
Thank you,


It is better to create a stable cell line with one of your plasmids. Then do a single transfection. A cotransfection would cause you to add too many antibiotics and may harm the cell.

Hope that works!