protein protein interaction - (Jul/24/2006 )
Dear all,
Are there any methods that can study protein protein interaction beside coimmunoprecipitation, overlaid and confocal microscopy? Researcher-friendly methods are prefered.
Thanks in advance.
Marco
-marco02-
QUOTE (marco02 @ Jul 24 2006, 09:39 AM)
Dear all,
Are there any methods that can study protein protein interaction beside coimmunoprecipitation, overlaid and confocal microscopy? Researcher-friendly methods are prefered.
Thanks in advance.
Marco
Are there any methods that can study protein protein interaction beside coimmunoprecipitation, overlaid and confocal microscopy? Researcher-friendly methods are prefered.
Thanks in advance.
Marco
FRET, BRET, SPR, ITC, FP... a lot of methods, but all very ambitious...
-ms-olli-
How about blue native electrophoresis?
-miss piggy-
QUOTE (marco02 @ Jul 24 2006, 08:39 PM)
Dear all,
Are there any methods that can study protein protein interaction beside coimmunoprecipitation, overlaid and confocal microscopy? Researcher-friendly methods are prefered.
Thanks in advance.
Marco
Are there any methods that can study protein protein interaction beside coimmunoprecipitation, overlaid and confocal microscopy? Researcher-friendly methods are prefered.
Thanks in advance.
Marco
Yeast two-hybrid will show if yor proteins interact, and by mutating one, you can define the interacting residues.
-swanny-