Problem with genomic DNA extracted from mammalian tissue using PCI method - (Jan/11/2006 )
I have been trying to standardize the DNA isolation method for my lab for some time.  I am using the old fasion PCI method (Current protocols in Molecular Biology).  I do not understnad what the problem is  .  The 260/280 values look good (between 1.7 and 2). But the value of 260/230 is much lower (0.2-1.5)  than expected (>2). Further, the samples when run on 0.8% agarose gel have vertical striataions.  
Any wrod of advise is welcome.
-Molishree-
Is it possible for you to upload a gel picture so we can figure out what is going on?
-rassen-
