Transfection Reagents - (Nov/15/2005 )
I noticed that most people use CaPO4 for transfection when they are making virus. Just being curious, is there a particular reason? Or it's just because CaPO4 is cheap.
I compared the transfection efficiency between CaPO4 and Fugene6, they are about the same.
-ucsdedu-
As far as I know, most people use CaPO4 on 293(T) cells because it is indeed cheaper and as you say has about the same transfection efficiency as Fugene6 (or other reagents) on these cells.
-vairus-
it's the cheapest reagents, and you get about 80% transfection efficiency for 293 cells with this reagent.
-liflaf1-