PMA induced U 937 differentiation to Macrophages - (Oct/05/2005 )
QUOTE (Babis @ Oct 5 2005, 10:56 PM)
Hi,
I Would like to ask if anybody knows in what concentration of PMA and in how many cells/mL U937 monocytic cell line is differentiated to macrophages. Yet, how many days are required for the differentiation to take place.
During differentiation should I expect any morphological change under microscopic observation or these cells are attached mantaining their round shape?
Thanks!
I Would like to ask if anybody knows in what concentration of PMA and in how many cells/mL U937 monocytic cell line is differentiated to macrophages. Yet, how many days are required for the differentiation to take place.
During differentiation should I expect any morphological change under microscopic observation or these cells are attached mantaining their round shape?
Thanks!
I use 10 ng/ml PMA in RPMI with antibiotic. Incubate for 48 hours. The cells attached well.
-syLoc-