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1. Oligo primers for shRNA construction - (reply: 1)
2. Antagomir design tool - (reply: 3)
3. Guide vs passenger strand sorting - (reply: 1)
4. How to explain this result? - (reply: 1)
5. Need an effective siRNA/ shRNA sequence for downregulating caveolin-1 - (reply: 2)
6. Higher mRNA level after RNAi? - (reply: 1)
7. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
8. question: RNAi for bacteria? - (reply: 3)
9. RNAi problem - Gene is knocked down, but not protein - (reply: 5)
10. Calculating siRNA Transfection Efficiency - (reply: 3)
11. miRNA extraction from cell-conditioned media? - (reply: 1)
12. si/shRNA sequences against eGFP - (reply: 1)
13. microRNA and 3' UTR - (reply: 2)
14. Designing primers for miRNA located on minus strand. - (reply: 2)
15. Need help with extracting miRNA from human serum using mirVana kit - (reply: 2)
16. siRNA time - (reply: 1)
17. mir30-based shrna cloning-mismatch - (reply: 1)
18. New RNAi video from Nature - (reply: 1)
19. checking whther there is any miRNA in introns of a specific gene. - (reply: 1)
20. What to do Post-transfection of cells with siRNA? - (reply: 2)
21. miRNA in serum - (reply: 1)
22. Difference between <Antisense RNA> & <RNAi>? - (reply: 1)
23. How to predict targets from a novel miRNA - (reply: 1)
24. Knock down efficiency - (reply: 2)
25. Let-7 and miR-143 miRNA? - (reply: 1)
26. protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
27. Targets of mirna* - (reply: 1)
28. Which company to buy siRNA from? - (reply: 1)
29. How to predict targets of unkown miRNAs in Drosophila - (reply: 2)
30. shRNA rescue with site directed mutagenesis - oligo design - (reply: 4)
2. Antagomir design tool - (reply: 3)
3. Guide vs passenger strand sorting - (reply: 1)
4. How to explain this result? - (reply: 1)
5. Need an effective siRNA/ shRNA sequence for downregulating caveolin-1 - (reply: 2)
6. Higher mRNA level after RNAi? - (reply: 1)
7. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
8. question: RNAi for bacteria? - (reply: 3)
9. RNAi problem - Gene is knocked down, but not protein - (reply: 5)
10. Calculating siRNA Transfection Efficiency - (reply: 3)
11. miRNA extraction from cell-conditioned media? - (reply: 1)
12. si/shRNA sequences against eGFP - (reply: 1)
13. microRNA and 3' UTR - (reply: 2)
14. Designing primers for miRNA located on minus strand. - (reply: 2)
15. Need help with extracting miRNA from human serum using mirVana kit - (reply: 2)
16. siRNA time - (reply: 1)
17. mir30-based shrna cloning-mismatch - (reply: 1)
18. New RNAi video from Nature - (reply: 1)
19. checking whther there is any miRNA in introns of a specific gene. - (reply: 1)
20. What to do Post-transfection of cells with siRNA? - (reply: 2)
21. miRNA in serum - (reply: 1)
22. Difference between <Antisense RNA> & <RNAi>? - (reply: 1)
23. How to predict targets from a novel miRNA - (reply: 1)
24. Knock down efficiency - (reply: 2)
25. Let-7 and miR-143 miRNA? - (reply: 1)
26. protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
27. Targets of mirna* - (reply: 1)
28. Which company to buy siRNA from? - (reply: 1)
29. How to predict targets of unkown miRNAs in Drosophila - (reply: 2)
30. shRNA rescue with site directed mutagenesis - oligo design - (reply: 4)