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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
1. BLOCK-iT Pol II miR RNAi system: Issues with generating stable knockdown - (reply: 1)
2. Stability of transient shRNA transfection - (reply: 1)
3. prediction of microRNA targets using the sequence - (reply: 1)
4. commercially available miRNA or small RNA? - (reply: 2)
5. could you help me with my stem loop RT primer? - (reply: 1)
6. Lentivirus transfection using RAW 264.7 cells - (reply: 1)
7. Gene silencing and miRNA isolation - (reply: 1)
8. non-radioactive detection of siRNA in plants - (reply: 4)
9. Subsequent DNA and siRNA transfection - (reply: 8)
10. Knockdown with shRNA not stable (Why?) - (reply: 3)
11. Reference genes in miRNAs - (reply: 3)
12. miR30 based shRNA in mouse cells - (reply: 3)
13. simultaneous knock down of two genes in a single cell line - (reply: 1)
14. help on an experiment - (reply: 1)
15. Pan-Cancer pattern of lncRNAs, miRNAs and their regulatory networks - (reply: 2)
16. A help for siRNA experiment - (reply: 2)
17. rna extraction protocol - (reply: 3)
18. Basic questions - (reply: 2)
19. miRNA-mediated competing endogenous RNAs (ceRNAs) networks - (reply: 4)
20. software for drawing small RNA molecules and nucleotides - (reply: 2)
21. miRNA knockdown in a mouse, tail vein injection (~50% KD) - (reply: 2)
22. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
23. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
24. Help with shRNAmiR30 based constructs (for a simple MD) - (reply: 3)
25. Question: How to differentiate siRNAs and mature miRNAs in plant by Northern blo - (reply: 3)
26. synthetic microRNA stability - (reply: 1)
27. siRNA detection......which strand shuld labelled - (reply: 2)
28. scrambeled miR sequence - (reply: 5)
29. miRNA overexpression troubleshooting - (reply: 2)
30. siRNA quantification using UV - (reply: 1)

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