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1. RNA journals - (reply: 4)
2. Detecting miRNA mimics by RT-PCR? - (reply: 4)
3. shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages - (reply: 1)
4. RNA alcohol/salt precipitation...low temperature - (reply: 1)
5. To small amount of cells used for shRNA transfection - (reply: 1)
6. shRNA interference using Lenti virus vector - (reply: 1)
7. miRNA quantification - (reply: 3)
8. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
9. Gene knockdown expression levels shRNAmirs - (reply: 2)
10. siRNA in E.Coli - (reply: 2)
11. siRNA resuspension in RNase-free water - (reply: 1)
12. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
13. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
14. microRNA promoter based expression vector? - (reply: 1)
15. miRNA expression profiling - (reply: 3)
16. microRNA validation in vivo - (reply: 1)
17. in vivo naked-mRNA delivery - (reply: 4)
18. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
19. Recommendation on siRNA transfection reagents - (reply: 2)
20. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
21. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
22. Transfection reagent stored at -20C - (reply: 2)
23. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
24. Synthetic microrna for normalization in serum samples - (reply: 1)
25. DNA and siRNA transfection and cytotoxicity - (reply: 1)
26. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
27. What is the best way to overexpress miRNAs? - (reply: 1)
28. Hiperfect siRNA in HT-29 cells help - (reply: 2)
29. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
30. Different amount of oligonucleotide - (reply: 1)
2. Detecting miRNA mimics by RT-PCR? - (reply: 4)
3. shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages - (reply: 1)
4. RNA alcohol/salt precipitation...low temperature - (reply: 1)
5. To small amount of cells used for shRNA transfection - (reply: 1)
6. shRNA interference using Lenti virus vector - (reply: 1)
7. miRNA quantification - (reply: 3)
8. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
9. Gene knockdown expression levels shRNAmirs - (reply: 2)
10. siRNA in E.Coli - (reply: 2)
11. siRNA resuspension in RNase-free water - (reply: 1)
12. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
13. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
14. microRNA promoter based expression vector? - (reply: 1)
15. miRNA expression profiling - (reply: 3)
16. microRNA validation in vivo - (reply: 1)
17. in vivo naked-mRNA delivery - (reply: 4)
18. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
19. Recommendation on siRNA transfection reagents - (reply: 2)
20. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
21. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
22. Transfection reagent stored at -20C - (reply: 2)
23. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
24. Synthetic microrna for normalization in serum samples - (reply: 1)
25. DNA and siRNA transfection and cytotoxicity - (reply: 1)
26. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
27. What is the best way to overexpress miRNAs? - (reply: 1)
28. Hiperfect siRNA in HT-29 cells help - (reply: 2)
29. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
30. Different amount of oligonucleotide - (reply: 1)