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Protein doesn't bind any column except Ni - (Mar/13/2009 )

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I regularly use resource Q on FPLC and the loaded protein always has imidazole. In fact I plan to hook up an anion exchange column in series with the Ni-NTA column and do both the purifications together to save time.

It works.



James Chou on May 7 2009, 10:14 PM said:

That was what I am going to say. You need to dialyze the medium after Ni purification.

-T C-
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