Protein doesn't bind any column except Ni - (Mar/13/2009 )
Hey,
I regularly use resource Q on FPLC and the loaded protein always has imidazole. In fact I plan to hook up an anion exchange column in series with the Ni-NTA column and do both the purifications together to save time.
It works.
Best,
TC
James Chou on May 7 2009, 10:14 PM said:
That was what I am going to say. You need to dialyze the medium after Ni purification.
-T C-