I am making at 5 mg/mL soln of Tetracycline, and am using EtOH per our most recent Molecular Cloning manual...but I am having trouble getting all of the solid dissolved. Should I be using 70 % ETOH? Any thoughts/suggestions would be appreciated!!

Ive made some Tet sol. a couple of times....be sure to use an analytical balance, and dissolve it in absolute ethanol (100%)....and shake it up like theres no tomorrow...itll take a while but it will go into solution...
Do not autoclave...its ready for use....
Cheers

slapolla on Mar 25 2005, 10:40 AM said:

i agree with matiascs. I do the same
chandima

People,

Err... How do we know when to use water or ethanol? We have so much stocks here that I have yet to prepare any antibiotic solutions.

dreamchaser_jc on Jan 29 2009, 09:54 AM said:

you use that in which it is soluble and stable and will not interfere with your purpose.

mdfenko on Jan 30 2009, 12:53 AM said:

Alright, got it!

According to Lab-facts (Roche) make the stock in Et-OH and the dilutions in liquid culture.

I believe the confusion is that two forms of tetracycline are marketed, the compound, and its hydrochloride. Tetracycline is soluble in EtOH, but not water. Tet-HCl is soluble in water. Dissolving Tet-HCl in 50% EtOH is what we like, since it keeps the solution at -20C liquid.

phage434 on Feb 13 2009, 08:42 AM said:

And from my experience Tet-HCL is not as soluble in Ethanol compared to water. Tet-HCL in Absolute EtOH is quite crappy in term of solubility.

Hi Guys,

I f we make tetracycline stock solution i absolute or 100% ethanol instead of water, dont u think that ethanol is dangerous/killer for E.Coli cells?

Yes i agree that not all tetracyline get dissolved , but i am still using it anyways by giving it a bit vortex.

Please advise.

Thanx.