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immunofluorescence - (Feb/09/2014 )

while doing immunofluorescence technique with frozen sections I mistakenly washed the sections with  5X PBS in stead of 1X PBS for 5 minutes. Will it do any harm to the sections? Can I continue my further work? Please suggest


The excess salt makes this a very stringent wash but won't damage the proteins you are trying to detect.  If it doesn't work, you may be able to re-probe with the antibodies.