immunofluorescence - (Feb/09/2014 )
while doing immunofluorescence technique with frozen sections I mistakenly washed the sections with 5X PBS in stead of 1X PBS for 5 minutes. Will it do any harm to the sections? Can I continue my further work? Please suggest
The excess salt makes this a very stringent wash but won't damage the proteins you are trying to detect. If it doesn't work, you may be able to re-probe with the antibodies.