Coating too much antigen in ELISA - (Oct/07/2013 )
I suppose to coat my ELISA plate with inactive virus; However, during the coating step, by miscalculation, I coating too much inactive virus. Will this affect by ELISA results? please help.....
Please pardon my English.. it is not my naive language.
Coating too much antigen can reduce signals a bit but usually is fine. If your assay is qualitative (yes/no) then there should not be any problem. Do you typicaly coat to saturate the well? What is the mass of virus you typically coat with? If you typically coat to saturate the well then all the miscalculation did was to waste antigen - it won't cause any problems to your assay.