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PBMC lysis protocol? - (Feb/13/2013 )

does anybody have a protocol for or experience in lysis of PBMC? I would like to use the proteins in an immunoassay. Is it possible to lyse it by sonication? Do I need a special lysis buffer?
thanks for your help.
best regards


most lyssis buffers will do. make sure you check the main page of there are plenty of protocols and recipes. If you still have questions come back to the forum and ask.