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Isolated lymphocytes activation - (Nov/28/2012 )

Im working with PBMC or purified lymphocytes and mi question is...

Im activating the cells with a-CD3/CD28 (1ug/ml) for 48 hrs...how much is the increase (proliferation) do i have to expect? Twice?...

Recently i have no proliferation or even less cells... In this days im going to check if they are really activated using CD69 by FACS...

-Curkobain-

More details are needed to help you with your issue:

1) What species is your PBMC source?
2) What lymphocyte have you purified (method please).
3) What is your anti-CD3/CD28 protocol for activation.
4) What is your readout for proliferation (good to confirm with CD69)?

-cellspecific.com-