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Zebrafish embedding - (Aug/05/2012 )


I'm trying to embed live zebrafish embryos (48 hpf) without using Tricaine/MS-222 (as it affects my results). I've tried embedding them in agarose, but it deprives the embryos of oxygen which is an improtant factor that affects my recordings. Does anyone know of any other method of immobolisation that would not deprive them of oxygen?


Patch clamp?