Electroporation in Pseudomonas aeruginosa - (Dec/19/2011 )
well, I would check them to be sure, but the changes are high they do not contain an insert.
But on the other hand: the non selective plates have the same kind of colonies? Then it might just be ok.... but are you sure you are growing them like you should, because you should see nice colonies normally.
My suggestion is don't waste time in maintaining this culture if you see same number of colonies in both
Thanks Vete and Kumar
Selected plate colonies were not very nice as it should be as usual. Then I discard all the plate and doing electroporation again. I will update you if I will get colonies.
Thanks again.
Ram