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Alexa Fluor 594 only showed with blue filter - (Nov/20/2011 )

We just got new mouse anti-elastin and Alexa Fluor 594 for donkey anti-mouse.
It is so weird that I can only see nice stained fibers with blue filter, not with yellow filter, where it is supposed to show.
Anyone has any ideal?

Thanks a lot


I suspect you are either seeing bleed through with your channels, or that you have some form of autofluorescence.