Xenograft in sv129 mice - (Jan/25/2011 )

Hello,

Can anyone suggest me what tumor cell line can I use for tumor xenograft model in SV129 background?

Thanks,

Botond

-Boti-

a line specific to the type of cancer you're studying?

-Fungus_Dreams-

a line specific to sv129

I'd like to grow a tumor in sv129 background mice

-Boti-

A xenograft would be from human to mouse (xeno=foreign), in which case you'll need immunocompromised mouse... For an allograft I would suggest B16 melanoma cells, they are quite efficient in primary and metastatic cancer establishment (but of Bl6 background). Depends on what you're looking for, I guess.

-Rsm-

Rsm on Fri Feb 11 14:07:28 2011 said:

If I'm right. B16 is of mouse origin and not human, right? The same goes for Lewis Lung arcinoma. My problem is that these are black6-derived.

In this case xeno means that the tumor is not of the host animal's origin. But thanks for your input.

-Boti-

Try to get some pure Sv129 ES cells. If you inject them, you'll have teratoma formation.

-Rsm-

Rsm on Mon Feb 14 18:18:45 2011 said:

Try to get some pure Sv129 ES cells. If you inject them, you'll have teratoma formation.

thank you

I will try that

-Boti-