EC50, ED50? - (Sep/15/2010 )

I'm making a primary neuronal cell culture line and I want to put several different concentrations of morphine on it to see if I can very the results, how do I find the EC50 or ED50 for morphine> Is this a good place to start, then steadily increase until about 10uM?

Thanks in advance!

hello ethan241,

well .. here i attach you a paper that shows you how to calculate the ED50 & the EC50 ...
wished i found a simpler one !

and here is a link to a paper computer programs for calculating LD50 or ED50

Best Wishes,
nightingale