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Protein Expression in stationary phase - (Mar/24/2010 )

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AbhayGene on Apr 8 2010, 12:15 AM said:

OK Fishdoc...Thank you so much!
Now, my question is how long the sds-page buffer lysed sample is stable in -20?
As I was away for some reasons, I lysed the samples in sds-page buffer,cooled and placed them in -20 for more than 10 days. Can I use those samples for western blot now? are they stable enough? :D

they should be fine. you may have to reboil. just ensure that there is no sds out of solution.

-mdfenko-

mdfenko on Apr 16 2010, 11:54 PM said:

AbhayGene on Apr 8 2010, 12:15 AM said:

OK Fishdoc...Thank you so much!
Now, my question is how long the sds-page buffer lysed sample is stable in -20?
As I was away for some reasons, I lysed the samples in sds-page buffer,cooled and placed them in -20 for more than 10 days. Can I use those samples for western blot now? are they stable enough? :huh:

they should be fine. you may have to reboil. just ensure that there is no sds out of solution.



Alright. Thanks

-AbhayGene-
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