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Neutrophil isolation - Isolation of PBMC and neutrophils from blood (Mar/22/2010 )

Hi,

I was wondering does anyone know if it possible to isolate neutrophils and PBMCs simultaneously from whole blood? So far I have only been isolating PBMCs using Ficoll.

Thanks.
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-bourkenm-

bourkenm on Mar 22 2010, 06:40 PM said:

Hi,

I was wondering does anyone know if it possible to isolate neutrophils and PBMCs simultaneously from whole blood? So far I have only been isolating PBMCs using Ficoll.

Thanks.

This is usually fairly straightforward using a single density centrifugation. A number of products are commercially available. For example:

Hope this helps
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-klinmed-