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CD 34 proliferation - (Mar/21/2010 )

Hi all,
I was trying to proliferate my CD34 cells for the last 3 weeks but the cells didn't grow, I am using HPGM media and different concentration of cytokines 50-100 ng/ml of SCF, TPO and FLT3, with different cell count 20,000-200,00.
Any recommendation?
Thank you in advance


What is the origin of your cells? i.e. Are they human, mouse...? How did you isolate them? has some good stuff on there for stem cell culture such as the SFEM.