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1. GST tag protein- soluble or insoluble??? help! - (reply: 2)
2. Rapid western blot buffer ?? - (reply: 1)
3. How to get soluble protein? - (reply: 3)
4. 'Standard salt buffer' for protein storage/biophysical techniques - (reply: 1)
5. Novel PTM site detection - (reply: 1)
6. Potassium phosphate buffer - (reply: 3)
7. Protein Isolation from mouse brain: problems - (reply: 2)
8. Expression level analysis at mRNA vs protein level - (reply: 6)
9. 【help】What could happen to this SDS-PAGE ? - (reply: 1)
10. Insoluble recombinant protein is expressed also with out IPTG - (reply: 4)
11. Far western blotting - (reply: 5)
12. citrate buffer pH 8 - (reply: 1)
13. What's the difference between megnatic beads and agarose beads to IP His-tag - (reply: 3)
14. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
15. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
16. getting rid of non specific bands in IP experiments - (reply: 7)
17. loading control antibodies - (reply: 1)
18. protein solubilization and quantitation after extraction using Trizol - (reply: 2)
19. detection of phosphorylation from cell pellet - (reply: 2)
20. how to make a recombinant enzyme refolded - (reply: 2)
21. Quantification of LDL Oxidation - (reply: 1)
22. Concentrating protein of a particular size - (reply: 1)
23. Removing a binding site - (reply: 5)
24. GFP 96-well black plates; plastic lid, sealer or paraffin oil to cover? - (reply: 3)
25. 96 well protein expression - (reply: 1)
26. IEF migration to middle of gel, no separation - (reply: 1)
27. P450 Activity Assay - (reply: 1)
28. Help with iTRAQ-SCX protocol - (reply: 1)
29. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
30. Problem: peptide (MW 2.3KDa) can't dialyze out - (reply: 5)
2. Rapid western blot buffer ?? - (reply: 1)
3. How to get soluble protein? - (reply: 3)
4. 'Standard salt buffer' for protein storage/biophysical techniques - (reply: 1)
5. Novel PTM site detection - (reply: 1)
6. Potassium phosphate buffer - (reply: 3)
7. Protein Isolation from mouse brain: problems - (reply: 2)
8. Expression level analysis at mRNA vs protein level - (reply: 6)
9. 【help】What could happen to this SDS-PAGE ? - (reply: 1)
10. Insoluble recombinant protein is expressed also with out IPTG - (reply: 4)
11. Far western blotting - (reply: 5)
12. citrate buffer pH 8 - (reply: 1)
13. What's the difference between megnatic beads and agarose beads to IP His-tag - (reply: 3)
14. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
15. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
16. getting rid of non specific bands in IP experiments - (reply: 7)
17. loading control antibodies - (reply: 1)
18. protein solubilization and quantitation after extraction using Trizol - (reply: 2)
19. detection of phosphorylation from cell pellet - (reply: 2)
20. how to make a recombinant enzyme refolded - (reply: 2)
21. Quantification of LDL Oxidation - (reply: 1)
22. Concentrating protein of a particular size - (reply: 1)
23. Removing a binding site - (reply: 5)
24. GFP 96-well black plates; plastic lid, sealer or paraffin oil to cover? - (reply: 3)
25. 96 well protein expression - (reply: 1)
26. IEF migration to middle of gel, no separation - (reply: 1)
27. P450 Activity Assay - (reply: 1)
28. Help with iTRAQ-SCX protocol - (reply: 1)
29. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
30. Problem: peptide (MW 2.3KDa) can't dialyze out - (reply: 5)