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Top : New Forum Archives (2009-): : Protein-and-Proteomics
1. Reverse coIP - (reply: 5)
2. N-terminus tagging and signalP prediction - (reply: 2)
3. how to predict transmembrane protein structure - (reply: 7)
4. slimy cell paste of recombinant e.coli - (reply: 3)
5. n=3 for pSILAC - (reply: 1)
6. Is there any significant difference between endogenous/ native promoter and cons - (reply: 1)
7. kDa - (reply: 1)
8. Salting out specific protein - (reply: 1)
9. Flow Cytometry vs Western Blotting: can't get the same result. Why? - (reply: 1)
10. Bradford assay which is the blank? - (reply: 7)
11. protein sequence mutagenesis - (reply: 2)
12. Mass Spectrometry help needed - (reply: 1)
13. Protein quantification using HPLC/fluorescence - (reply: 3)
14. Effect on protein of low pH? - (reply: 1)
15. BCA microplate assay with 595 nm filter? - (reply: 1)
16. Cannot figure this dot blot thing out - (reply: 2)
17. Stability of a typical protein at 4oC? - (reply: 1)
18. Uses of ion exchange chromatography? - (reply: 1)
19. Friendly antibody buffer to prevent precipitation? - (reply: 3)
20. Use of fluorescent stain on IEF gels - (reply: 1)
21. Use of speedvac to dry in-gel peptide digest before sending for LCMS - (reply: 2)
22. doublets on 2D gel - (reply: 4)
23. RNA later and 2D SDS PAGE - (reply: 3)
24. Peptide quantification - (reply: 4)
25. Affinity purification glycine elution chemistry - (reply: 3)
26. Zeba buffer exchange low protein yield - (reply: 6)
27. iTRAQ or ELISA? - (reply: 1)
28. 2D Quant kit - (reply: 2)
29. 2D PAGE and Western blotting - (reply: 1)
30. How to detect specific-protein producing cells? - (reply: 8)