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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. pKD1 based vector for K. lactis expression - (reply: 1)
2. HSP70 contamination after Nickel purification - (reply: 3)
3. Use of urea in solubilizing protein and its aftermath - (reply: 5)
4. GST fusion protein - two dominant bands - (reply: 3)
5. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
6. Increasing protein solubility in general - (reply: 3)
7. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
8. Best protocol to solubilize bacterial fusion proteins? - (reply: 5)
9. Doubt on HisTag protein purification miniprep - (reply: 3)
10. "Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)
11. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
12. after gelfiltration need to concentrate peptide mix - (reply: 4)
13. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
14. Protein that used to express really well stopped expressing. - (reply: 2)
15. Determining Cofactor for Inactive Enzyme - (reply: 6)
16. study protein expression in bacteria - (reply: 1)
17. total protein measurement from insect body - (reply: 1)
18. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
19. Large-scale cell growth and protein expression - (reply: 1)
20. Reduce protein concentration without reduced enzyme activity - (reply: 3)
21. consistent expression of overlarge proteins - (reply: 1)
22. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
23. Is AS pp + HIC phisically possible for all proteins? - (reply: 2)
24. storage conditions for purified protein - (reply: 2)
25. Need to include protease inhibitor in IP wash buffer? - (reply: 1)
26. Heat shock vs eletroporation - (reply: 6)
27. lysate protein concentration - (reply: 1)
28. Cloned gene in pET system not being expressed - (reply: 10)
29. recombinant protein expression- surface or internal? - (reply: 3)
30. His-tag protein not binding to resin - (reply: 9)

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