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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. How to obtain a non-cellular protein in its natural form? - (reply: 4)
2. Protein expression issue - (reply: 4)
3. GLUT1 and GLUT4 expression in serum - (reply: 2)
4. Time period of sodiun lauryl sarcosine - (reply: 1)
5. Outer membrane protein extraction - (reply: 5)
6. Virus vs protein purification - (reply: 3)
7. S. aureus lysis protocol for LC-MS/MS - (reply: 2)
8. How to make antibody based affinity column for protein purification - (reply: 4)
9. Agarose beads IP troubles - (reply: 7)
10. Problem with Rosetta gami B protein expression!!!! - (reply: 1)
11. bacterial surface protein extraction - (reply: 1)
12. peptide concentation clearification - (reply: 1)
13. peptide size either in Da or kDa - (reply: 1)
14. determination of protein concentation - (reply: 1)
15. Protein concentration in mM - (reply: 3)
16. polb cloned in pET not expressed - (reply: 2)
17. protein purification from agarose beads using immunoprecipation kit - (reply: 2)
18. Fab antibody fragment purification - (reply: 2)
19. Arctic Express Cpn60 Antibody - (reply: 1)
20. AKTA FPLC - UV instability - (reply: 4)
21. Re-purification of His-tagged protein over IMAC - (reply: 2)
22. Anti-Penta His antibody detecting extra bands - (reply: 2)
23. Pros and Cons of refolding proteins - (reply: 3)
24. pKD1 based vector for K. lactis expression - (reply: 2)
25. HSP70 contamination after Nickel purification - (reply: 3)
26. Use of urea in solubilizing protein and its aftermath - (reply: 5)
27. GST fusion protein - two dominant bands - (reply: 7)
28. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
29. Increasing protein solubility in general - (reply: 3)
30. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)

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