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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. A single point mutation enhanced protein expression but attenuated its function - (reply: 4)
2. Why is my His-SUMO tag being cleaved during purification? - (reply: 1)
3. How to obtain a non-cellular protein in its natural form? - (reply: 4)
4. Protein expression issue - (reply: 4)
5. GLUT1 and GLUT4 expression in serum - (reply: 2)
6. Time period of sodiun lauryl sarcosine - (reply: 1)
7. Outer membrane protein extraction - (reply: 5)
8. Virus vs protein purification - (reply: 3)
9. S. aureus lysis protocol for LC-MS/MS - (reply: 2)
10. How to make antibody based affinity column for protein purification - (reply: 4)
11. Agarose beads IP troubles - (reply: 7)
12. Problem with Rosetta gami B protein expression!!!! - (reply: 1)
13. bacterial surface protein extraction - (reply: 1)
14. peptide concentation clearification - (reply: 1)
15. peptide size either in Da or kDa - (reply: 1)
16. determination of protein concentation - (reply: 1)
17. Protein concentration in mM - (reply: 3)
18. polb cloned in pET not expressed - (reply: 2)
19. protein purification from agarose beads using immunoprecipation kit - (reply: 2)
20. Fab antibody fragment purification - (reply: 2)
21. Arctic Express Cpn60 Antibody - (reply: 1)
22. AKTA FPLC - UV instability - (reply: 4)
23. Re-purification of His-tagged protein over IMAC - (reply: 2)
24. Anti-Penta His antibody detecting extra bands - (reply: 2)
25. Pros and Cons of refolding proteins - (reply: 3)
26. pKD1 based vector for K. lactis expression - (reply: 2)
27. HSP70 contamination after Nickel purification - (reply: 3)
28. Use of urea in solubilizing protein and its aftermath - (reply: 5)
29. GST fusion protein - two dominant bands - (reply: 7)
30. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)

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