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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. protein purification from agarose beads using immunoprecipation kit - (reply: 1)
2. Fab antibody fragment purification - (reply: 2)
3. Arctic Express Cpn60 Antibody - (reply: 1)
4. AKTA FPLC - UV instability - (reply: 4)
5. Re-purification of His-tagged protein over IMAC - (reply: 2)
6. Anti-Penta His antibody detecting extra bands - (reply: 2)
7. Pros and Cons of refolding proteins - (reply: 3)
8. pKD1 based vector for K. lactis expression - (reply: 2)
9. HSP70 contamination after Nickel purification - (reply: 3)
10. Use of urea in solubilizing protein and its aftermath - (reply: 5)
11. GST fusion protein - two dominant bands - (reply: 3)
12. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
13. Increasing protein solubility in general - (reply: 3)
14. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
15. Best protocol to solubilize bacterial fusion proteins? - (reply: 5)
16. Doubt on HisTag protein purification miniprep - (reply: 3)
17. "Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)
18. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
19. after gelfiltration need to concentrate peptide mix - (reply: 4)
20. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
21. Protein that used to express really well stopped expressing. - (reply: 2)
22. Determining Cofactor for Inactive Enzyme - (reply: 6)
23. study protein expression in bacteria - (reply: 1)
24. total protein measurement from insect body - (reply: 1)
25. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
26. Large-scale cell growth and protein expression - (reply: 1)
27. Reduce protein concentration without reduced enzyme activity - (reply: 3)
28. consistent expression of overlarge proteins - (reply: 1)
29. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
30. Is AS pp + HIC phisically possible for all proteins? - (reply: 2)

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