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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Time period of sodiun lauryl sarcosine - (reply: 1)
2. Outer membrane protein extraction - (reply: 5)
3. Virus vs protein purification - (reply: 3)
4. S. aureus lysis protocol for LC-MS/MS - (reply: 2)
5. How to make antibody based affinity column for protein purification - (reply: 4)
6. Agarose beads IP troubles - (reply: 7)
7. Problem with Rosetta gami B protein expression!!!! - (reply: 1)
8. bacterial surface protein extraction - (reply: 1)
9. peptide concentation clearification - (reply: 1)
10. peptide size either in Da or kDa - (reply: 1)
11. determination of protein concentation - (reply: 1)
12. Protein concentration in mM - (reply: 3)
13. polb cloned in pET not expressed - (reply: 2)
14. protein purification from agarose beads using immunoprecipation kit - (reply: 2)
15. Fab antibody fragment purification - (reply: 2)
16. Arctic Express Cpn60 Antibody - (reply: 1)
17. AKTA FPLC - UV instability - (reply: 4)
18. Re-purification of His-tagged protein over IMAC - (reply: 2)
19. Anti-Penta His antibody detecting extra bands - (reply: 2)
20. Pros and Cons of refolding proteins - (reply: 3)
21. pKD1 based vector for K. lactis expression - (reply: 2)
22. HSP70 contamination after Nickel purification - (reply: 3)
23. Use of urea in solubilizing protein and its aftermath - (reply: 5)
24. GST fusion protein - two dominant bands - (reply: 7)
25. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
26. Increasing protein solubility in general - (reply: 3)
27. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
28. Best protocol to solubilize bacterial fusion proteins? - (reply: 5)
29. Doubt on HisTag protein purification miniprep - (reply: 3)
30. "Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)

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