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1. NL and L? - (reply: 2)
2. e coli vector and host selection - (reply: 1)
3. Double tagging - (reply: 1)
4. Purifying recombinant protein from pellet? - (reply: 2)
5. Co-expression of 2 plasmids in E.coli - (reply: 1)
6. Protein ladder - (reply: 1)
7. Trouble producing stable cell lines - (reply: 2)
8. Can anyone help me to identify what line is that??? - (reply: 4)
9. enrichment with wheat germ agglutinin (WGA) - (reply: 1)
10. membrane protein expression - (reply: 1)
11. Magnesium acetate in protein lysates - (reply: 1)
12. Protease Inhibitor Cocktails - (reply: 3)
13. E. coli cells refuse to grow! - (reply: 2)
14. Extra bands after protein expression/purification - (reply: 4)
15. Better protein Expression? - (reply: 2)
16. Expression of protein with pI 8.2 - (reply: 1)
17. Methionine for protein expression - (reply: 3)
18. Alternatives to benzonase? - (reply: 1)
19. Protein Expression problems - (reply: 4)
20. Advice on soluble aggregate problem - (reply: 2)
21. problem in expressing A DNA binding protein - (reply: 1)
22. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
23. histone proteins extraction and 2D - (reply: 1)
24. Nickel Column Purficiation - (reply: 2)
25. Sarkosyl and Ni-NTA - (reply: 1)
26. Insoluble Protein purification - (reply: 1)
27. Problems with detergents and Ni-NTA - (reply: 5)
28. Bradford method - (reply: 4)
29. protein extraction from pirmary keratinocytes - (reply: 2)
30. How to purify unstable protein? - (reply: 10)
2. e coli vector and host selection - (reply: 1)
3. Double tagging - (reply: 1)
4. Purifying recombinant protein from pellet? - (reply: 2)
5. Co-expression of 2 plasmids in E.coli - (reply: 1)
6. Protein ladder - (reply: 1)
7. Trouble producing stable cell lines - (reply: 2)
8. Can anyone help me to identify what line is that??? - (reply: 4)
9. enrichment with wheat germ agglutinin (WGA) - (reply: 1)
10. membrane protein expression - (reply: 1)
11. Magnesium acetate in protein lysates - (reply: 1)
12. Protease Inhibitor Cocktails - (reply: 3)
13. E. coli cells refuse to grow! - (reply: 2)
14. Extra bands after protein expression/purification - (reply: 4)
15. Better protein Expression? - (reply: 2)
16. Expression of protein with pI 8.2 - (reply: 1)
17. Methionine for protein expression - (reply: 3)
18. Alternatives to benzonase? - (reply: 1)
19. Protein Expression problems - (reply: 4)
20. Advice on soluble aggregate problem - (reply: 2)
21. problem in expressing A DNA binding protein - (reply: 1)
22. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
23. histone proteins extraction and 2D - (reply: 1)
24. Nickel Column Purficiation - (reply: 2)
25. Sarkosyl and Ni-NTA - (reply: 1)
26. Insoluble Protein purification - (reply: 1)
27. Problems with detergents and Ni-NTA - (reply: 5)
28. Bradford method - (reply: 4)
29. protein extraction from pirmary keratinocytes - (reply: 2)
30. How to purify unstable protein? - (reply: 10)