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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 1)
2. Loss of protein expression by transformed bacteria - (reply: 3)
3. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
4. after protein purification - (reply: 4)
5. Tryple express vs scraping - (reply: 2)
6. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
7. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
8. protein expression site - (reply: 3)
9. Beads pull down only free GST - (reply: 14)
10. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
11. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
12. protein expression - (reply: 2)
13. Anion Exchange Buffers - (reply: 2)
14. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
15. about sds-page buffers - (reply: 1)
16. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
17. Best epitope for IF with a poor-expresser - (reply: 2)
18. Collagen binding protein expression E. coli His-tag - (reply: 1)
19. pet-32 - (reply: 3)
20. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
21. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
22. Question about MBP purification - (reply: 2)
23. unexpected protein bands - (reply: 2)
24. Urea effect on mice spleen cells - (reply: 1)
25. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)
26. Validating Recombinant Protein - (reply: 1)
27. Sonication of cells in ultrasound bath, without probe - (reply: 3)
28. Proteinase extraction and purification - (reply: 1)
29. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
30. EGFP expression - (reply: 1)

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