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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Fab antibody fragment purification - (reply: 1)
2. Arctic Express Cpn60 Antibody - (reply: 1)
3. AKTA FPLC - UV instability - (reply: 4)
4. Re-purification of His-tagged protein over IMAC - (reply: 2)
5. Anti-Penta His antibody detecting extra bands - (reply: 1)
6. Pros and Cons of refolding proteins - (reply: 3)
7. pKD1 based vector for K. lactis expression - (reply: 2)
8. HSP70 contamination after Nickel purification - (reply: 3)
9. Use of urea in solubilizing protein and its aftermath - (reply: 5)
10. GST fusion protein - two dominant bands - (reply: 3)
11. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
12. Increasing protein solubility in general - (reply: 3)
13. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
14. Best protocol to solubilize bacterial fusion proteins? - (reply: 5)
15. Doubt on HisTag protein purification miniprep - (reply: 3)
16. "Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)
17. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
18. after gelfiltration need to concentrate peptide mix - (reply: 4)
19. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
20. Protein that used to express really well stopped expressing. - (reply: 2)
21. Determining Cofactor for Inactive Enzyme - (reply: 6)
22. study protein expression in bacteria - (reply: 1)
23. total protein measurement from insect body - (reply: 1)
24. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
25. Large-scale cell growth and protein expression - (reply: 1)
26. Reduce protein concentration without reduced enzyme activity - (reply: 3)
27. consistent expression of overlarge proteins - (reply: 1)
28. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
29. Is AS pp + HIC phisically possible for all proteins? - (reply: 2)
30. storage conditions for purified protein - (reply: 2)

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