Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. polb cloned in pET not expressed - (reply: 2)
2. protein purification from agarose beads using immunoprecipation kit - (reply: 1)
3. Fab antibody fragment purification - (reply: 2)
4. Arctic Express Cpn60 Antibody - (reply: 1)
5. AKTA FPLC - UV instability - (reply: 4)
6. Re-purification of His-tagged protein over IMAC - (reply: 2)
7. Anti-Penta His antibody detecting extra bands - (reply: 2)
8. Pros and Cons of refolding proteins - (reply: 3)
9. pKD1 based vector for K. lactis expression - (reply: 2)
10. HSP70 contamination after Nickel purification - (reply: 3)
11. Use of urea in solubilizing protein and its aftermath - (reply: 5)
12. GST fusion protein - two dominant bands - (reply: 3)
13. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
14. Increasing protein solubility in general - (reply: 3)
15. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
16. Best protocol to solubilize bacterial fusion proteins? - (reply: 5)
17. Doubt on HisTag protein purification miniprep - (reply: 3)
18. "Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)
19. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
20. after gelfiltration need to concentrate peptide mix - (reply: 4)
21. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
22. Protein that used to express really well stopped expressing. - (reply: 2)
23. Determining Cofactor for Inactive Enzyme - (reply: 6)
24. study protein expression in bacteria - (reply: 1)
25. total protein measurement from insect body - (reply: 1)
26. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
27. Large-scale cell growth and protein expression - (reply: 1)
28. Reduce protein concentration without reduced enzyme activity - (reply: 3)
29. consistent expression of overlarge proteins - (reply: 1)
30. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22