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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Protein expression issue - (reply: 3)
2. GLUT1 and GLUT4 expression in serum - (reply: 2)
3. Time period of sodiun lauryl sarcosine - (reply: 1)
4. Outer membrane protein extraction - (reply: 5)
5. Virus vs protein purification - (reply: 3)
6. S. aureus lysis protocol for LC-MS/MS - (reply: 2)
7. How to make antibody based affinity column for protein purification - (reply: 4)
8. Agarose beads IP troubles - (reply: 7)
9. Problem with Rosetta gami B protein expression!!!! - (reply: 1)
10. bacterial surface protein extraction - (reply: 1)
11. peptide concentation clearification - (reply: 1)
12. peptide size either in Da or kDa - (reply: 1)
13. determination of protein concentation - (reply: 1)
14. Protein concentration in mM - (reply: 3)
15. polb cloned in pET not expressed - (reply: 2)
16. protein purification from agarose beads using immunoprecipation kit - (reply: 2)
17. Fab antibody fragment purification - (reply: 2)
18. Arctic Express Cpn60 Antibody - (reply: 1)
19. AKTA FPLC - UV instability - (reply: 4)
20. Re-purification of His-tagged protein over IMAC - (reply: 2)
21. Anti-Penta His antibody detecting extra bands - (reply: 2)
22. Pros and Cons of refolding proteins - (reply: 3)
23. pKD1 based vector for K. lactis expression - (reply: 2)
24. HSP70 contamination after Nickel purification - (reply: 3)
25. Use of urea in solubilizing protein and its aftermath - (reply: 5)
26. GST fusion protein - two dominant bands - (reply: 7)
27. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
28. Increasing protein solubility in general - (reply: 3)
29. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
30. Best protocol to solubilize bacterial fusion proteins? - (reply: 5)

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