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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Determining Cofactor for Inactive Enzyme - (reply: 6)
2. study protein expression in bacteria - (reply: 1)
3. total protein measurement from insect body - (reply: 1)
4. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
5. Large-scale cell growth and protein expression - (reply: 1)
6. Reduce protein concentration without reduced enzyme activity - (reply: 3)
7. consistent expression of overlarge proteins - (reply: 1)
8. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
9. Is AS pp + HIC phisically possible for all proteins? - (reply: 2)
10. storage conditions for purified protein - (reply: 2)
11. Need to include protease inhibitor in IP wash buffer? - (reply: 1)
12. Heat shock vs eletroporation - (reply: 6)
13. lysate protein concentration - (reply: 1)
14. Cloned gene in pET system not being expressed - (reply: 10)
15. recombinant protein expression- surface or internal? - (reply: 3)
16. His-tag protein not binding to resin - (reply: 9)
17. Protein localisation - IHC - (reply: 1)
18. Collagen Tipe I filtration - (reply: 1)
19. Removal of cleaved peptide after Protease cleavage - (reply: 4)
20. Bradford positive but no Protein on SDS PAGE - (reply: 4)
21. Disadvantage of crossflow filtration - (reply: 3)
22. Help with Superdex200 increase Column - (reply: 1)
23. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
24. Loss of protein expression by transformed bacteria - (reply: 4)
25. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
26. after protein purification - (reply: 4)
27. Tryple express vs scraping - (reply: 2)
28. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
29. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
30. recombinant protein vs native protein - (reply: 1)

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