Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. AKTA FPLC - UV instability - (reply: 4)
2. Re-purification of His-tagged protein over IMAC - (reply: 2)
3. Anti-Penta His antibody detecting extra bands - (reply: 1)
4. Pros and Cons of refolding proteins - (reply: 3)
5. pKD1 based vector for K. lactis expression - (reply: 2)
6. HSP70 contamination after Nickel purification - (reply: 3)
7. Use of urea in solubilizing protein and its aftermath - (reply: 5)
8. GST fusion protein - two dominant bands - (reply: 3)
9. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
10. Increasing protein solubility in general - (reply: 3)
11. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
12. Best protocol to solubilize bacterial fusion proteins? - (reply: 5)
13. Doubt on HisTag protein purification miniprep - (reply: 3)
14. "Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)
15. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
16. after gelfiltration need to concentrate peptide mix - (reply: 4)
17. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
18. Protein that used to express really well stopped expressing. - (reply: 2)
19. Determining Cofactor for Inactive Enzyme - (reply: 6)
20. study protein expression in bacteria - (reply: 1)
21. total protein measurement from insect body - (reply: 1)
22. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
23. Large-scale cell growth and protein expression - (reply: 1)
24. Reduce protein concentration without reduced enzyme activity - (reply: 3)
25. consistent expression of overlarge proteins - (reply: 1)
26. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
27. Is AS pp + HIC phisically possible for all proteins? - (reply: 2)
28. storage conditions for purified protein - (reply: 2)
29. Need to include protease inhibitor in IP wash buffer? - (reply: 1)
30. Heat shock vs eletroporation - (reply: 6)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22