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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 1)
2. protein expression with and without induction - (reply: 1)
3. Please help me :Protein expression and purification - (reply: 1)
4. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
5. genotype of e coli strain for protein expression - (reply: 2)
6. Problems with detection of protein expressed in yeast - (reply: 1)
7. thionin - (reply: 1)
8. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
9. secretome and zymograms - (reply: 1)
10. Protein Refolding - Precipitation during dialysis - (reply: 3)
11. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
12. Problem with calculations for enzyme activity - (reply: 2)
13. problem with sds-page - (reply: 8)
14. Ammonium sulfate fractionalization help - (reply: 1)
15. heme protein precipitation - (reply: 4)
16. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 3)
17. regarding protein purification using 8m urea and sds page - (reply: 2)
18. how do you inactivate His-tag affinity beads?? - (reply: 4)
19. Protein Purification from mammalian cells - (reply: 1)
20. DNA cleanup after linearization, before transformation? - (reply: 3)
21. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
22. predict VLP formation - (reply: 1)
23. need help for transfection to synchronized cells - (reply: 4)
24. Pulldown of polyubiquitinated proteins - (reply: 1)
25. membrane protein expression - (reply: 2)
26. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
27. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
28. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
29. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
30. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)

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