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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. RIPA buffer for IP and western - (reply: 1)
2. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
3. CO- IP for nuclear protein - (reply: 4)
4. Trichloroacetic Acid precipitation of proteins - (reply: 2)
5. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)
6. protein expression with and without induction - (reply: 1)
7. Please help me :Protein expression and purification - (reply: 1)
8. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
9. genotype of e coli strain for protein expression - (reply: 2)
10. Problems with detection of protein expressed in yeast - (reply: 1)
11. thionin - (reply: 1)
12. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
13. secretome and zymograms - (reply: 1)
14. Protein Refolding - Precipitation during dialysis - (reply: 3)
15. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
16. Problem with calculations for enzyme activity - (reply: 2)
17. problem with sds-page - (reply: 8)
18. Ammonium sulfate fractionalization help - (reply: 1)
19. heme protein precipitation - (reply: 4)
20. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 3)
21. regarding protein purification using 8m urea and sds page - (reply: 2)
22. how do you inactivate His-tag affinity beads?? - (reply: 4)
23. Protein Purification from mammalian cells - (reply: 1)
24. DNA cleanup after linearization, before transformation? - (reply: 3)
25. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
26. predict VLP formation - (reply: 1)
27. need help for transfection to synchronized cells - (reply: 4)
28. Pulldown of polyubiquitinated proteins - (reply: 1)
29. membrane protein expression - (reply: 2)
30. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)

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