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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. How to make antibody based affinity column for protein purification - (reply: 4)
2. Agarose beads IP troubles - (reply: 7)
3. Problem with Rosetta gami B protein expression!!!! - (reply: 1)
4. bacterial surface protein extraction - (reply: 1)
5. peptide concentation clearification - (reply: 1)
6. peptide size either in Da or kDa - (reply: 1)
7. determination of protein concentation - (reply: 1)
8. Protein concentration in mM - (reply: 3)
9. polb cloned in pET not expressed - (reply: 2)
10. protein purification from agarose beads using immunoprecipation kit - (reply: 2)
11. Fab antibody fragment purification - (reply: 2)
12. Arctic Express Cpn60 Antibody - (reply: 1)
13. AKTA FPLC - UV instability - (reply: 4)
14. Re-purification of His-tagged protein over IMAC - (reply: 2)
15. Anti-Penta His antibody detecting extra bands - (reply: 2)
16. Pros and Cons of refolding proteins - (reply: 3)
17. pKD1 based vector for K. lactis expression - (reply: 2)
18. HSP70 contamination after Nickel purification - (reply: 3)
19. Use of urea in solubilizing protein and its aftermath - (reply: 5)
20. GST fusion protein - two dominant bands - (reply: 7)
21. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
22. Increasing protein solubility in general - (reply: 3)
23. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
24. Best protocol to solubilize bacterial fusion proteins? - (reply: 5)
25. Doubt on HisTag protein purification miniprep - (reply: 3)
26. "Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)
27. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
28. after gelfiltration need to concentrate peptide mix - (reply: 4)
29. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
30. Protein that used to express really well stopped expressing. - (reply: 2)

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