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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Increasing protein solubility in general - (reply: 3)
2. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
3. Best protocol to solubilize bacterial fusion proteins? - (reply: 5)
4. Doubt on HisTag protein purification miniprep - (reply: 3)
5. "Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)
6. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
7. after gelfiltration need to concentrate peptide mix - (reply: 4)
8. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
9. Protein that used to express really well stopped expressing. - (reply: 2)
10. Determining Cofactor for Inactive Enzyme - (reply: 6)
11. study protein expression in bacteria - (reply: 1)
12. total protein measurement from insect body - (reply: 1)
13. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
14. Large-scale cell growth and protein expression - (reply: 1)
15. Reduce protein concentration without reduced enzyme activity - (reply: 3)
16. consistent expression of overlarge proteins - (reply: 1)
17. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
18. Is AS pp + HIC phisically possible for all proteins? - (reply: 2)
19. storage conditions for purified protein - (reply: 2)
20. Need to include protease inhibitor in IP wash buffer? - (reply: 1)
21. Heat shock vs eletroporation - (reply: 6)
22. lysate protein concentration - (reply: 1)
23. Cloned gene in pET system not being expressed - (reply: 10)
24. recombinant protein expression- surface or internal? - (reply: 3)
25. His-tag protein not binding to resin - (reply: 9)
26. Protein localisation - IHC - (reply: 1)
27. Collagen Tipe I filtration - (reply: 1)
28. Removal of cleaved peptide after Protease cleavage - (reply: 4)
29. Bradford positive but no Protein on SDS PAGE - (reply: 4)
30. Disadvantage of crossflow filtration - (reply: 3)

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