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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. How to eliminate inherent proteins contamination during protein purification? - (reply: 1)
2. A single point mutation enhanced protein expression but attenuated its function - (reply: 4)
3. Why is my His-SUMO tag being cleaved during purification? - (reply: 1)
4. How to obtain a non-cellular protein in its natural form? - (reply: 4)
5. Protein expression issue - (reply: 4)
6. GLUT1 and GLUT4 expression in serum - (reply: 2)
7. Time period of sodiun lauryl sarcosine - (reply: 1)
8. Outer membrane protein extraction - (reply: 5)
9. Virus vs protein purification - (reply: 3)
10. S. aureus lysis protocol for LC-MS/MS - (reply: 2)
11. How to make antibody based affinity column for protein purification - (reply: 4)
12. Agarose beads IP troubles - (reply: 7)
13. Problem with Rosetta gami B protein expression!!!! - (reply: 1)
14. bacterial surface protein extraction - (reply: 1)
15. peptide concentation clearification - (reply: 1)
16. peptide size either in Da or kDa - (reply: 1)
17. determination of protein concentation - (reply: 1)
18. Protein concentration in mM - (reply: 3)
19. polb cloned in pET not expressed - (reply: 2)
20. protein purification from agarose beads using immunoprecipation kit - (reply: 2)
21. Fab antibody fragment purification - (reply: 2)
22. Arctic Express Cpn60 Antibody - (reply: 1)
23. AKTA FPLC - UV instability - (reply: 4)
24. Re-purification of His-tagged protein over IMAC - (reply: 2)
25. Anti-Penta His antibody detecting extra bands - (reply: 2)
26. Pros and Cons of refolding proteins - (reply: 3)
27. pKD1 based vector for K. lactis expression - (reply: 2)
28. HSP70 contamination after Nickel purification - (reply: 3)
29. Use of urea in solubilizing protein and its aftermath - (reply: 5)
30. GST fusion protein - two dominant bands - (reply: 7)

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