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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Trouble transforming pET28a in E. coli BL21(DE3) - (reply: 5)
2. Choosing a common exon in GOI to design your guides - (reply: 1)
3. Designing Crispr guides and cloning into appropriate vectors - (reply: 1)
4. plasmid purification - (reply: 4)
5. Cloning protein of interest into lentiviral vector - help - I have no experience - (reply: 20)
6. Insert in frame - (reply: 1)
7. PCRed-vector approach to tag switching - (reply: 1)
8. High copy vs low copy plasmids: which one causes less toxicity? - (reply: 2)
9. Blunt-end ligation: what if the vector and insert are both phosphorylated? - (reply: 4)
10. low copy number plasmid purification kit - (reply: 4)
11. Neomycin/Kanamycin Resistant gene - (reply: 2)
12. Plasmid analysis in limited conditions - (reply: 6)
13. Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
14. Troubleshooting with restriction enzymes during cloning - (reply: 5)
15. Transformation - (reply: 3)
16. Plasmid distributors for Luciferase Assay? - (reply: 2)
17. Ligate a very small insert into a vector - (reply: 3)
18. Restriction enzyme - (reply: 1)
19. Plasmid dna size - (reply: 3)
20. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
21. Express two different membrane proteins on the same promoter - (reply: 1)
22. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
23. [Overexpression] Without upstream transcription terminator - (reply: 4)
24. How large need homology arms to be? - (reply: 1)
25. Ampicillin LB agar reheating and reusing - (reply: 2)
26. Using pB2GW7,0 - (reply: 2)
27. propagating commercial chemically competent cells - (reply: 2)
28. Plasmid purification for Gibson Assembly - (reply: 2)
29. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
30. TA cloning..Urgent Help!! - (reply: 4)