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Top : New Forum Archives (2009-): : Molecular-Cloning
1. TA cloning..Urgent Help!! - (reply: 3)
2. Can someone please explain this to me? - (reply: 1)
3. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
4. TetA as a counter-selection marker - (reply: 1)
5. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
6. pET32b+ vector issue - (reply: 1)
7. Subcloning issue - (reply: 2)
8. Splicing DNA fragments - (reply: 1)
9. Need to add 12 bp at the beginning of the sequence - (reply: 4)
10. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
11. Problem with possible leaky expression in cloning - (reply: 4)
12. Luciferase assay cloning - (reply: 2)
13. Selecting bacteria with Puromycin - (reply: 1)
14. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
15. How stable is double digested plasmid - (reply: 7)
16. Problems with ligation - (reply: 2)
17. Problem with restriction digestion of cloned PCR product - (reply: 5)
18. SLIC Cloning Failure - (reply: 2)
19. Failed Gibson transformation - (reply: 7)
20. how to check for intron-exon junctions - (reply: 2)
21. Lower Plasmid Yields - (reply: 5)
22. Cleaning loading dye for PCR sequencing - (reply: 1)
23. Using SDS for DNA electrophoresis - (reply: 3)
24. Bacterial knockout by double crossover - (reply: 4)
25. Polymerase mixture for blunt ended fragments - (reply: 2)
26. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
27. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
28. Question in gateway system, LR reaction - (reply: 5)
29. Problem with subcloning genescript synthesized genes - (reply: 2)
30. Flourescence visualization - (reply: 2)