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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Faster method to screeen for positive colonies. - (reply: 7)
2. 260/230 ratio, does it matter? - (reply: 4)
3. Double digestion problem with PUCsp vector - (reply: 1)
4. How much plasmid for transformation? - (reply: 7)
5. RE digestion and ligation troubleshooting. - (reply: 1)
6. replicating a mammalian expression vector in bacteria - (reply: 3)
7. Cloning of an unknown gene - (reply: 3)
8. cloning problems using in-fusion kit - (reply: 5)
9. Mutation in one chain of homodimer - (reply: 3)
10. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
11. Alternative competent cells for QuikChange kit - (reply: 2)
12. Ligation Mistake - (reply: 4)
13. Cloning - Ligation problem - (reply: 3)
14. How to clone a 2A peptide between two ORFs? - (reply: 2)
15. preparing a vector for transfection - (reply: 2)
16. Ligation of two PCR products - (reply: 1)
17. TA Cloning - (reply: 11)
18. Troubleshootting lentiviral transduction. - (reply: 3)
19. Trouble with PCR using ligation mix as template? - (reply: 3)
20. Suitable ratio of vector and insert in cloning - (reply: 5)
21. The right lentivirus plasmid for overexpression in human cells - (reply: 3)
22. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
23. plasmid repositories - (reply: 2)
24. How to insert PreScission protease into a vector - (reply: 1)
25. Why the insert become shorter after inserting into plasmid? - (reply: 2)
26. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
27. Thermo scientific T4 DNA ligase product help - (reply: 3)
28. Cloning a big insert in a small vector - (reply: 2)
29. Transformation E.Coli help - (reply: 2)
30. Exonucleases that won't harm circular dsDNA - (reply: 1)