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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Neomycin/Kanamycin Resistant gene - (reply: 2)
2. Plasmid analysis in limited conditions - (reply: 4)
3. Troubleshooting with restriction enzymes during cloning - (reply: 4)
4. Transformation - (reply: 2)
5. Plasmid distributors for Luciferase Assay? - (reply: 2)
6. Ligate a very small insert into a vector - (reply: 3)
7. Restriction enzyme - (reply: 1)
8. Plasmid dna size - (reply: 3)
9. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
10. Express two different membrane proteins on the same promoter - (reply: 1)
11. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
12. [Overexpression] Without upstream transcription terminator - (reply: 4)
13. How large need homology arms to be? - (reply: 1)
14. Ampicillin LB agar reheating and reusing - (reply: 2)
15. Using pB2GW7,0 - (reply: 2)
16. propagating commercial chemically competent cells - (reply: 2)
17. Plasmid purification for Gibson Assembly - (reply: 2)
18. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
19. TA cloning..Urgent Help!! - (reply: 4)
20. Can someone please explain this to me? - (reply: 2)
21. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
22. TetA as a counter-selection marker - (reply: 1)
23. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
24. pET32b+ vector issue - (reply: 1)
25. Subcloning issue - (reply: 2)
26. Splicing DNA fragments - (reply: 2)
27. Need to add 12 bp at the beginning of the sequence - (reply: 4)
28. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
29. Problem with possible leaky expression in cloning - (reply: 4)
30. Luciferase assay cloning - (reply: 2)