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Molecular-Cloning
1.
NZY+Broth can be replaced by LB Broth in mutagenesis exp -
(reply: 1)
2.
oligo(dt) 15 vs random primers -
(reply: 1)
3.
Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? -
(reply: 2)
4.
Why don't I get the expected entry clones when I use the gateway system?? -
(reply: 2)
5.
Need help verifying promotor of a PUC19 based plasmid -
(reply: 1)
6.
cloning into Pgem t easy -
(reply: 1)
7.
How to get pure protein of a gene -
(reply: 3)
8.
How to insert genes into chromosome of E. coli -
(reply: 1)
9.
Gene synthesis -
(reply: 2)
10.
Pls help me with this plasmid!!! -
(reply: 1)
11.
Problems with Restriction Digest Results -
(reply: 7)
12.
Methods of plasmid transformation? only heat shock? -
(reply: 1)
13.
Digestion -
(reply: 2)
14.
Digestion of Genomic DNA -
(reply: 1)
15.
Isolation of a Gene of Interest for Subcloning -
(reply: 8)
16.
E.coli grow on neg. control -
(reply: 1)
17.
Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins -
(reply: 1)
18.
Need help troubleshooting digest. -
(reply: 1)
19.
Competent cells question: JM101 vs DH5alpha -
(reply: 2)
20.
How to avoid plasmid instability -
(reply: 4)
21.
Question Help -
(reply: 1)
22.
Shear-induced damage of plasmid by vortexing -
(reply: 1)
23.
Problems with blunt end ligation -
(reply: 3)
24.
Problem with Transforming E. coli DH10Bac -
(reply: 7)
25.
Blunt-ligating dephosphorylated insert into phosphorylated vector -
(reply: 5)
26.
Heat inactivate ligase -
(reply: 5)
27.
DMSO stock of overnight bacterial cultures -
(reply: 1)
28.
Recommended competent cell for transformation of large plasmids? -
(reply: 4)
29.
Prolem in screening of hygromycin concentration -
(reply: 1)
30.
Primers for Introduction of new restriction sites to a vector -
(reply: 3)
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