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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
2. How to find promoter sequence ... - (reply: 2)
3. Restriction double digest - (reply: 1)
4. Choice of AMP 100 versus AMP 50 - (reply: 1)
5. Why are there two origin of replications in plasmids - (reply: 3)
6. How can we expand molecular cloning further? - (reply: 3)
7. Subtle change in insert size after cloning - (reply: 6)
8. lactic acid bacteria - cloning - (reply: 2)
9. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
10. All I get is supercoiled DNA... - (reply: 5)
11. Help Quesion - (reply: 1)
12. No colonies after self Circularization ligation - (reply: 2)
13. Clean ligation controls but no insert - (reply: 12)
14. ratios insert/vector - (reply: 3)
15. Can't isolate plasmid from cloned E.coli - (reply: 3)
16. Genomic DNA quantification using the NanoDrop - (reply: 3)
17. Competent cells with Hanahan's method - (reply: 2)
18. cloning issues - (reply: 3)
19. Is important the direction of IRES-GFP in cloning? - (reply: 1)
20. What are the error-rates of the TA cloning Kits? - (reply: 8)
21. Can mutations be created during cloning? - (reply: 7)
22. confusing with where to put primers and which ordination - (reply: 4)
23. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
24. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
25. RBS - (reply: 1)
26. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
27. Gel for my ligation - (reply: 1)
28. Amplifying from pcr products - (reply: 7)
29. Design the primers - (reply: 10)
30. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)