Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1. Restriction enzyme - (reply: 1)
2. Plasmid dna size - (reply: 2)
3. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
4. Express two different membrane proteins on the same promoter - (reply: 1)
5. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
6. [Overexpression] Without upstream transcription terminator - (reply: 4)
7. How large need homology arms to be? - (reply: 1)
8. Ampicillin LB agar reheating and reusing - (reply: 2)
9. Using pB2GW7,0 - (reply: 2)
10. propagating commercial chemically competent cells - (reply: 2)
11. Plasmid purification for Gibson Assembly - (reply: 2)
12. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
13. TA cloning..Urgent Help!! - (reply: 4)
14. Can someone please explain this to me? - (reply: 2)
15. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
16. TetA as a counter-selection marker - (reply: 1)
17. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
18. pET32b+ vector issue - (reply: 1)
19. Subcloning issue - (reply: 2)
20. Splicing DNA fragments - (reply: 2)
21. Need to add 12 bp at the beginning of the sequence - (reply: 4)
22. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
23. Problem with possible leaky expression in cloning - (reply: 4)
24. Luciferase assay cloning - (reply: 2)
25. Selecting bacteria with Puromycin - (reply: 1)
26. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
27. How stable is double digested plasmid - (reply: 7)
28. Problems with ligation - (reply: 2)
29. Problem with restriction digestion of cloned PCR product - (reply: 5)
30. SLIC Cloning Failure - (reply: 2)