1. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 1)
2. oligo(dt) 15 vs random primers - (reply: 1)
3. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
4. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
5. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
6. cloning into Pgem t easy - (reply: 1)
7. How to get pure protein of a gene - (reply: 3)
8. How to insert genes into chromosome of E. coli - (reply: 1)
9. Gene synthesis - (reply: 2)
10. Pls help me with this plasmid!!! - (reply: 1)
11. Problems with Restriction Digest Results - (reply: 7)
12. Methods of plasmid transformation? only heat shock? - (reply: 1)
13. Digestion - (reply: 2)
14. Digestion of Genomic DNA - (reply: 1)
15. Isolation of a Gene of Interest for Subcloning - (reply: 8)
16. E.coli grow on neg. control - (reply: 1)
17. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
18. Need help troubleshooting digest. - (reply: 1)
19. Competent cells question: JM101 vs DH5alpha - (reply: 2)
20. How to avoid plasmid instability - (reply: 4)
21. Question Help - (reply: 1)
22. Shear-induced damage of plasmid by vortexing - (reply: 1)
23. Problems with blunt end ligation - (reply: 3)
24. Problem with Transforming E. coli DH10Bac - (reply: 7)
25. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 5)
26. Heat inactivate ligase - (reply: 5)
27. DMSO stock of overnight bacterial cultures - (reply: 1)
28. Recommended competent cell for transformation of large plasmids? - (reply: 4)
29. Prolem in screening of hygromycin concentration - (reply: 1)
30. Primers for Introduction of new restriction sites to a vector - (reply: 3)

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