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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Weird results with bacterial transformation - (reply: 5)
2. Site Directed Mutagenesis - no product - (reply: 4)
3. Problems sequencing ligation product - (reply: 5)
4. Faster method to screeen for positive colonies. - (reply: 7)
5. 260/230 ratio, does it matter? - (reply: 4)
6. Double digestion problem with PUCsp vector - (reply: 1)
7. How much plasmid for transformation? - (reply: 8)
8. RE digestion and ligation troubleshooting. - (reply: 1)
9. replicating a mammalian expression vector in bacteria - (reply: 3)
10. Cloning of an unknown gene - (reply: 3)
11. cloning problems using in-fusion kit - (reply: 5)
12. Mutation in one chain of homodimer - (reply: 3)
13. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
14. Alternative competent cells for QuikChange kit - (reply: 2)
15. Ligation Mistake - (reply: 4)
16. Cloning - Ligation problem - (reply: 3)
17. How to clone a 2A peptide between two ORFs? - (reply: 2)
18. preparing a vector for transfection - (reply: 2)
19. Ligation of two PCR products - (reply: 1)
20. TA Cloning - (reply: 11)
21. Troubleshootting lentiviral transduction. - (reply: 3)
22. Trouble with PCR using ligation mix as template? - (reply: 3)
23. Suitable ratio of vector and insert in cloning - (reply: 5)
24. The right lentivirus plasmid for overexpression in human cells - (reply: 3)
25. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
26. plasmid repositories - (reply: 2)
27. How to insert PreScission protease into a vector - (reply: 1)
28. Why the insert become shorter after inserting into plasmid? - (reply: 2)
29. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
30. Thermo scientific T4 DNA ligase product help - (reply: 3)