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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Plasmid dna size - (reply: 2)
2. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
3. Express two different membrane proteins on the same promoter - (reply: 1)
4. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
5. [Overexpression] Without upstream transcription terminator - (reply: 4)
6. How large need homology arms to be? - (reply: 1)
7. Ampicillin LB agar reheating and reusing - (reply: 2)
8. Using pB2GW7,0 - (reply: 2)
9. propagating commercial chemically competent cells - (reply: 2)
10. Plasmid purification for Gibson Assembly - (reply: 2)
11. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
12. TA cloning..Urgent Help!! - (reply: 4)
13. Can someone please explain this to me? - (reply: 2)
14. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
15. TetA as a counter-selection marker - (reply: 1)
16. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
17. pET32b+ vector issue - (reply: 1)
18. Subcloning issue - (reply: 2)
19. Splicing DNA fragments - (reply: 2)
20. Need to add 12 bp at the beginning of the sequence - (reply: 4)
21. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
22. Problem with possible leaky expression in cloning - (reply: 4)
23. Luciferase assay cloning - (reply: 2)
24. Selecting bacteria with Puromycin - (reply: 1)
25. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
26. How stable is double digested plasmid - (reply: 7)
27. Problems with ligation - (reply: 2)
28. Problem with restriction digestion of cloned PCR product - (reply: 5)
29. SLIC Cloning Failure - (reply: 2)
30. Failed Gibson transformation - (reply: 7)