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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Blunt-end ligation: what if the vector and insert are both phosphorylated? - (reply: 4)
2. low copy number plasmid purification kit - (reply: 3)
3. Neomycin/Kanamycin Resistant gene - (reply: 2)
4. Plasmid analysis in limited conditions - (reply: 4)
5. Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
6. Troubleshooting with restriction enzymes during cloning - (reply: 4)
7. Transformation - (reply: 3)
8. Plasmid distributors for Luciferase Assay? - (reply: 2)
9. Ligate a very small insert into a vector - (reply: 3)
10. Restriction enzyme - (reply: 1)
11. Plasmid dna size - (reply: 3)
12. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
13. Express two different membrane proteins on the same promoter - (reply: 1)
14. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
15. [Overexpression] Without upstream transcription terminator - (reply: 4)
16. How large need homology arms to be? - (reply: 1)
17. Ampicillin LB agar reheating and reusing - (reply: 2)
18. Using pB2GW7,0 - (reply: 2)
19. propagating commercial chemically competent cells - (reply: 2)
20. Plasmid purification for Gibson Assembly - (reply: 2)
21. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
22. TA cloning..Urgent Help!! - (reply: 4)
23. Can someone please explain this to me? - (reply: 2)
24. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
25. TetA as a counter-selection marker - (reply: 1)
26. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
27. pET32b+ vector issue - (reply: 1)
28. Subcloning issue - (reply: 2)
29. Splicing DNA fragments - (reply: 2)
30. Need to add 12 bp at the beginning of the sequence - (reply: 4)