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Top : New Forum Archives (2009-): : Molecular-Cloning
1. preparing a vector for transfection - (reply: 2)
2. Ligation of two PCR products - (reply: 1)
3. TA Cloning - (reply: 11)
4. Troubleshootting lentiviral transduction. - (reply: 3)
5. Trouble with PCR using ligation mix as template? - (reply: 3)
6. Suitable ratio of vector and insert in cloning - (reply: 5)
7. The right lentivirus plasmid for overexpression in human cells - (reply: 3)
8. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
9. plasmid repositories - (reply: 2)
10. How to insert PreScission protease into a vector - (reply: 1)
11. Why the insert become shorter after inserting into plasmid? - (reply: 2)
12. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
13. Thermo scientific T4 DNA ligase product help - (reply: 3)
14. Cloning a big insert in a small vector - (reply: 2)
15. Transformation E.Coli help - (reply: 2)
16. Exonucleases that won't harm circular dsDNA - (reply: 1)
17. How to produce transgenic mice? - (reply: 10)
18. No colonies on plate after liagtion - (reply: 7)
19. Question: Can I cut out rb glob PA Terminator - (reply: 1)
20. Low Yield Miniprep - (reply: 5)
21. question about Clonetech Retro-X Tet-on system - (reply: 3)
22. quiaquick gel extraction troubleshooting - (reply: 1)
23. Insert direction in TA cloning - (reply: 2)
24. Transformation in Staphylococcus epidermidis - (reply: 1)
25. Plasmids recombining - (reply: 3)
26. troubleshooting for a simple SDM - (reply: 2)
27. Should I proceed with clone selection? - (reply: 2)
28. Need help in codon optimization... - (reply: 1)
29. transformation - (reply: 2)
30. problem in sequencing of gene - (reply: 3)