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Top : New Forum Archives (2009-): : Molecular-Cloning
1. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
2. Express two different membrane proteins on the same promoter - (reply: 1)
3. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
4. [Overexpression] Without upstream transcription terminator - (reply: 4)
5. How large need homology arms to be? - (reply: 1)
6. Ampicillin LB agar reheating and reusing - (reply: 2)
7. Using pB2GW7,0 - (reply: 2)
8. propagating commercial chemically competent cells - (reply: 2)
9. Plasmid purification for Gibson Assembly - (reply: 2)
10. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
11. TA cloning..Urgent Help!! - (reply: 4)
12. Can someone please explain this to me? - (reply: 2)
13. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
14. TetA as a counter-selection marker - (reply: 1)
15. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
16. pET32b+ vector issue - (reply: 1)
17. Subcloning issue - (reply: 2)
18. Splicing DNA fragments - (reply: 2)
19. Need to add 12 bp at the beginning of the sequence - (reply: 4)
20. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
21. Problem with possible leaky expression in cloning - (reply: 4)
22. Luciferase assay cloning - (reply: 2)
23. Selecting bacteria with Puromycin - (reply: 1)
24. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
25. How stable is double digested plasmid - (reply: 7)
26. Problems with ligation - (reply: 2)
27. Problem with restriction digestion of cloned PCR product - (reply: 5)
28. SLIC Cloning Failure - (reply: 2)
29. Failed Gibson transformation - (reply: 7)
30. how to check for intron-exon junctions - (reply: 2)