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Top : New Forum Archives (2009-): : Molecular-Cloning
1. How large need homology arms to be? - (reply: 1)
2. Ampicillin LB agar reheating and reusing - (reply: 2)
3. Using pB2GW7,0 - (reply: 2)
4. propagating commercial chemically competent cells - (reply: 2)
5. Plasmid purification for Gibson Assembly - (reply: 2)
6. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
7. TA cloning..Urgent Help!! - (reply: 4)
8. Can someone please explain this to me? - (reply: 1)
9. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
10. TetA as a counter-selection marker - (reply: 1)
11. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
12. pET32b+ vector issue - (reply: 1)
13. Subcloning issue - (reply: 2)
14. Splicing DNA fragments - (reply: 2)
15. Need to add 12 bp at the beginning of the sequence - (reply: 4)
16. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
17. Problem with possible leaky expression in cloning - (reply: 4)
18. Luciferase assay cloning - (reply: 2)
19. Selecting bacteria with Puromycin - (reply: 1)
20. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
21. How stable is double digested plasmid - (reply: 7)
22. Problems with ligation - (reply: 2)
23. Problem with restriction digestion of cloned PCR product - (reply: 5)
24. SLIC Cloning Failure - (reply: 2)
25. Failed Gibson transformation - (reply: 7)
26. how to check for intron-exon junctions - (reply: 2)
27. Lower Plasmid Yields - (reply: 5)
28. Cleaning loading dye for PCR sequencing - (reply: 1)
29. Using SDS for DNA electrophoresis - (reply: 3)
30. Bacterial knockout by double crossover - (reply: 4)