Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1. High copy vs low copy plasmids: which one causes less toxicity? - (reply: 1)
2. Blunt-end ligation: what if the vector and insert are both phosphorylated? - (reply: 4)
3. low copy number plasmid purification kit - (reply: 4)
4. Neomycin/Kanamycin Resistant gene - (reply: 2)
5. Plasmid analysis in limited conditions - (reply: 6)
6. Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
7. Troubleshooting with restriction enzymes during cloning - (reply: 4)
8. Transformation - (reply: 3)
9. Plasmid distributors for Luciferase Assay? - (reply: 2)
10. Ligate a very small insert into a vector - (reply: 3)
11. Restriction enzyme - (reply: 1)
12. Plasmid dna size - (reply: 3)
13. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
14. Express two different membrane proteins on the same promoter - (reply: 1)
15. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
16. [Overexpression] Without upstream transcription terminator - (reply: 4)
17. How large need homology arms to be? - (reply: 1)
18. Ampicillin LB agar reheating and reusing - (reply: 2)
19. Using pB2GW7,0 - (reply: 2)
20. propagating commercial chemically competent cells - (reply: 2)
21. Plasmid purification for Gibson Assembly - (reply: 2)
22. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
23. TA cloning..Urgent Help!! - (reply: 4)
24. Can someone please explain this to me? - (reply: 2)
25. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
26. TetA as a counter-selection marker - (reply: 1)
27. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
28. pET32b+ vector issue - (reply: 1)
29. Subcloning issue - (reply: 2)
30. Splicing DNA fragments - (reply: 2)