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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Using pB2GW7,0 - (reply: 2)
2. propagating commercial chemically competent cells - (reply: 2)
3. Plasmid purification for Gibson Assembly - (reply: 2)
4. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
5. TA cloning..Urgent Help!! - (reply: 4)
6. Can someone please explain this to me? - (reply: 1)
7. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
8. TetA as a counter-selection marker - (reply: 1)
9. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
10. pET32b+ vector issue - (reply: 1)
11. Subcloning issue - (reply: 2)
12. Splicing DNA fragments - (reply: 2)
13. Need to add 12 bp at the beginning of the sequence - (reply: 4)
14. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
15. Problem with possible leaky expression in cloning - (reply: 4)
16. Luciferase assay cloning - (reply: 2)
17. Selecting bacteria with Puromycin - (reply: 1)
18. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
19. How stable is double digested plasmid - (reply: 7)
20. Problems with ligation - (reply: 2)
21. Problem with restriction digestion of cloned PCR product - (reply: 5)
22. SLIC Cloning Failure - (reply: 2)
23. Failed Gibson transformation - (reply: 7)
24. how to check for intron-exon junctions - (reply: 2)
25. Lower Plasmid Yields - (reply: 5)
26. Cleaning loading dye for PCR sequencing - (reply: 1)
27. Using SDS for DNA electrophoresis - (reply: 3)
28. Bacterial knockout by double crossover - (reply: 4)
29. Polymerase mixture for blunt ended fragments - (reply: 2)
30. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)