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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Gel for my ligation - (reply: 1)
2. Amplifying from pcr products - (reply: 4)
3. Design the primers - (reply: 10)
4. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
5. How two fragments joint together when doing fusion PCR? - (reply: 4)
6. Working backwards from a construct? - (reply: 1)
7. disappearing bases - (reply: 4)
8. Mutagenesis PCR - (reply: 9)
9. Digest two sites located next to each other - (reply: 5)
10. cDNA Library Construction by using kits and Sequencing - (reply: 1)
11. Looking for software that permutes codons to find new restriction sites - (reply: 4)
12. Transforming dna from agarose gel - (reply: 3)
13. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
14. ccdb toxin - (reply: 15)
15. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
16. annealing oligos - (reply: 3)
17. Does a restriction enzyme cut at a related site? - (reply: 5)
18. primers for cloning - (reply: 1)
19. Vector-insert ratio - (reply: 6)
20. Cloning contamination - (reply: 3)
21. plasmid construction - (reply: 2)
22. primer design with restriction enzyme - (reply: 1)
23. vector propagation in E coli - (reply: 1)
24. Restriction to remove background - (reply: 4)
25. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
26. Restriction and ligation time interval - (reply: 1)
27. Problem shrna cloning, bad sequencing results - (reply: 1)
28. Lentivirus copy number - (reply: 7)
29. Genomic -Lactamase? Blue White screening not working - (reply: 1)
30. direction of the expression units in multi-gene vectors - (reply: 1)