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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Designing Crispr guides and cloning into appropriate vectors - (reply: 1)
2. plasmid purification - (reply: 4)
3. Cloning protein of interest into lentiviral vector - help - I have no experience - (reply: 19)
4. Insert in frame - (reply: 1)
5. PCRed-vector approach to tag switching - (reply: 1)
6. High copy vs low copy plasmids: which one causes less toxicity? - (reply: 2)
7. Blunt-end ligation: what if the vector and insert are both phosphorylated? - (reply: 4)
8. low copy number plasmid purification kit - (reply: 4)
9. Neomycin/Kanamycin Resistant gene - (reply: 2)
10. Plasmid analysis in limited conditions - (reply: 6)
11. Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
12. Troubleshooting with restriction enzymes during cloning - (reply: 5)
13. Transformation - (reply: 3)
14. Plasmid distributors for Luciferase Assay? - (reply: 2)
15. Ligate a very small insert into a vector - (reply: 3)
16. Restriction enzyme - (reply: 1)
17. Plasmid dna size - (reply: 3)
18. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
19. Express two different membrane proteins on the same promoter - (reply: 1)
20. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
21. [Overexpression] Without upstream transcription terminator - (reply: 4)
22. How large need homology arms to be? - (reply: 1)
23. Ampicillin LB agar reheating and reusing - (reply: 2)
24. Using pB2GW7,0 - (reply: 2)
25. propagating commercial chemically competent cells - (reply: 2)
26. Plasmid purification for Gibson Assembly - (reply: 2)
27. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
28. TA cloning..Urgent Help!! - (reply: 4)
29. Can someone please explain this to me? - (reply: 2)
30. Problem with polyA retrotranscription: I have truncated products - (reply: 1)