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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Can't isolate plasmid from cloned E.coli - (reply: 2)
2. Genomic DNA quantification using the NanoDrop - (reply: 3)
3. Competent cells with Hanahan's method - (reply: 2)
4. cloning issues - (reply: 3)
5. Is important the direction of IRES-GFP in cloning? - (reply: 1)
6. What are the error-rates of the TA cloning Kits? - (reply: 7)
7. Can mutations be created during cloning? - (reply: 7)
8. confusing with where to put primers and which ordination - (reply: 4)
9. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
10. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
11. RBS - (reply: 1)
12. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
13. Gel for my ligation - (reply: 1)
14. Amplifying from pcr products - (reply: 7)
15. Design the primers - (reply: 10)
16. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
17. How two fragments joint together when doing fusion PCR? - (reply: 4)
18. Working backwards from a construct? - (reply: 1)
19. disappearing bases - (reply: 4)
20. Mutagenesis PCR - (reply: 9)
21. Digest two sites located next to each other - (reply: 5)
22. cDNA Library Construction by using kits and Sequencing - (reply: 1)
23. Looking for software that permutes codons to find new restriction sites - (reply: 4)
24. Transforming dna from agarose gel - (reply: 3)
25. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
26. ccdb toxin - (reply: 15)
27. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
28. annealing oligos - (reply: 3)
29. Does a restriction enzyme cut at a related site? - (reply: 5)
30. primers for cloning - (reply: 1)