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New Forum Archives (2009-)
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Molecular-Biology
1.
Bacterial Operon Knockout - Technique Help -
(reply: 1)
2.
Double Linker ligation is it possible? -
(reply: 1)
3.
Multiple bands in eluted PCR product -
(reply: 1)
4.
pCCL vector map -
(reply: 2)
5.
BAC miniprep-high nanodrop value but NO BAND -
(reply: 2)
6.
multiple bands for genomic DNA extraction -
(reply: 2)
7.
Getting genomic DNA for PCR -
(reply: 4)
8.
What would happened if I forgot to wash embyos with PBST after fixed them with 4 -
(reply: 2)
9.
RNA transcription from T3 promoter problem -
(reply: 1)
10.
How to purify RNA from a handful number of cells? -
(reply: 5)
11.
Conventional PCR -
(reply: 1)
12.
ISH on fluorescing cells -
(reply: 1)
13.
PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig -
(reply: 2)
14.
PCR product loss on cleanup -
(reply: 2)
15.
Phase separation during DNA extraction -
(reply: 5)
16.
cloning of a long gene using cDNA -
(reply: 1)
17.
RACE product confirmation -
(reply: 1)
18.
RNA purification from urine samples -
(reply: 1)
19.
Specific region of the promoter? -
(reply: 1)
20.
weird problem in cloning!! -
(reply: 4)
21.
PLEASE HELP: phenol-chloroform extraction and EtOH precipitation problem -
(reply: 4)
22.
Cloning HELP- Possible Primer issues -
(reply: 6)
23.
No plasmid after a maxi prep mystery -
(reply: 4)
24.
Repurifying DNA -
(reply: 3)
25.
RE-PCR -
(reply: 1)
26.
Is some amount of RNA obligatory for qRT-PCR? -
(reply: 5)
27.
Purifying RNA from Solution with RNEasy? -
(reply: 2)
28.
Western blot does not match IF -
(reply: 2)
29.
Problem with expressing genes using tac promoter -
(reply: 4)
30.
What can ssDNA ladder do? -
(reply: 6)
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