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Top : New Forum Archives (2009-): : Molecular-Biology
1. Odd issue converting mutiplex PCR to single reactions - (reply: 1)
2. What is your favorite method for getting inserts into vectors? - (reply: 3)
3. Possibly a stupid question. - (reply: 3)
4. Dilution help - (reply: 1)
5. Question about CaCl2 and CaCl2 - (reply: 5)
6. Preservation of RNA/cDNA samples - (reply: 1)
7. Cycloheximide concentration - (reply: 2)
8. Cell cycle - (reply: 2)
9. Design of <=40bp primers adding restriction sites and also Gibson Assembly o - (reply: 1)
10. Very low yields for >10kb fragment gel extractions - (reply: 1)
11. DNA extraction with phenol-chloroform - DNA stuck in phenol layer? - (reply: 1)
12. Cloning of big inserts - (reply: 1)
13. Primer design HELP! - (reply: 4)
14. wnt/beta catenin signalling - (reply: 1)
15. Using RNA minikit for DNA extraction - (reply: 4)
16. Getting terrible grainy background in immunofluorescence experiment - (reply: 5)
17. Purification of linearized AT-rich DNA for transfection - (reply: 4)
18. DNA decontamination chemistry - (reply: 1)
19. Why is the smearing becoming different in the polyacrylamide gel than the agaros - (reply: 2)
20. Site directed mutagenesis stopped working. - (reply: 6)
21. transfection - (reply: 2)
22. cell invasion assay - (reply: 1)
23. a half less of plasmid copy number in the solution resulting from ddPCR than qPC - (reply: 1)
24. Need to run gel after miniprep before digest? - (reply: 1)
25. Good diluents for RNA - (reply: 1)
26. solution preparation - (reply: 1)
27. concentration of starting material - (reply: 1)
28. Qiagen ATL, AL, AW1, AW2 recipes? - (reply: 1)
29. site directed mutagenesis - (reply: 1)
30. DNA concentration - (reply: 1)