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Top : New Forum Archives (2009-): : Molecular-Biology
1. Design of <=40bp primers adding restriction sites and also Gibson Assembly o - (reply: 1)
2. Very low yields for >10kb fragment gel extractions - (reply: 1)
3. DNA extraction with phenol-chloroform - DNA stuck in phenol layer? - (reply: 1)
4. Cloning of big inserts - (reply: 1)
5. Primer design HELP! - (reply: 4)
6. Using RNA minikit for DNA extraction - (reply: 4)
7. Getting terrible grainy background in immunofluorescence experiment - (reply: 5)
8. Purification of linearized AT-rich DNA for transfection - (reply: 4)
9. DNA decontamination chemistry - (reply: 1)
10. Why is the smearing becoming different in the polyacrylamide gel than the agaros - (reply: 2)
11. Site directed mutagenesis stopped working. - (reply: 6)
12. transfection - (reply: 2)
13. cell invasion assay - (reply: 1)
14. a half less of plasmid copy number in the solution resulting from ddPCR than qPC - (reply: 1)
15. Need to run gel after miniprep before digest? - (reply: 1)
16. Good diluents for RNA - (reply: 1)
17. solution preparation - (reply: 1)
18. concentration of starting material - (reply: 1)
19. Qiagen ATL, AL, AW1, AW2 recipes? - (reply: 1)
20. site directed mutagenesis - (reply: 1)
21. DNA concentration - (reply: 1)
22. what‘s phage display principle - (reply: 1)
23. pGEM T Easy subcloning - (reply: 1)
24. autoantibody - (reply: 1)
25. lentiviral transduction in BLCL cells - (reply: 3)
26. Are relaxed plasmids origin of rep sensitive to bp substitution in RNA I/II sequ - (reply: 5)
27. Is there a way to drive translation of ONLY in vitro transcribed mRNA? - (reply: 1)
28. Possible to change SimpleProbe/Melting curve to TaqMan or Scorpion? - (reply: 1)
29. Alternate cell line not giving same results as first - (reply: 1)
30. HTP DNA and RNA extraction options??? - (reply: 2)