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New Forum Archives (2009-)
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Immunology
1.
What is ELISA? -
(reply: 1)
2.
fixation and immunostaining of neurons after live-cell imaging -
(reply: 1)
3.
Are hybridoma germline DNA and hyrbidoma cDNA sequences identical? -
(reply: 2)
4.
Protocol for a solid phase sandwich ELISA? -
(reply: 1)
5.
monoclonal antibody immunization schedule -
(reply: 1)
6.
Aberrant (apparently) nuclear immunostaining for VEGF in Glial cells -
(reply: 2)
7.
Help: Lateral Flow Assay -
(reply: 1)
8.
Immunofluorescence Staining of Cells -
(reply: 1)
9.
How divergent sequences is foreign enough for antibody production? -
(reply: 1)
10.
Age of paraffin embedded tissue -
(reply: 1)
11.
Can I do immunohistochemistry on this?? -
(reply: 1)
12.
Costaining species-specific antibody question -
(reply: 4)
13.
Immunofluorescence - unspecific staining -
(reply: 9)
14.
DAB staining and GFP -
(reply: 1)
15.
Frozen vs paraffin: Confocal microscopy -
(reply: 3)
16.
protocol for immunostaining primery cells -
(reply: 5)
17.
Hybridmas- does anyone have experience with them? Please help -
(reply: 2)
18.
IgG control -
(reply: 6)
19.
Positive control ELISA -
(reply: 3)
20.
Hybridoma cells not secreting antibody -
(reply: 3)
21.
Immunocytochemistry - Primary Ab incubation time -
(reply: 2)
22.
Immunohistochemistry: What am I looking at? -
(reply: 2)
23.
Immunocytochemistry with peroxidase -
(reply: 1)
24.
Please explain me the use of congenic strains -
(reply: 4)
25.
No protein in cytoplasmic extract -
(reply: 1)
26.
Cytokine Assay: supernatants to pool or not to pool? -
(reply: 1)
27.
Is the T7 tag sequence immunogenic (from pET plasmid) -
(reply: 2)
28.
What is PANVAC Vaccine? -
(reply: 1)
29.
Calibration curve? -
(reply: 5)
30.
Polyclonal antibody production using SDS-PAGE gel fragment -
(reply: 3)
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